Int J Med Sci 2022; 19(3):499-510. doi:10.7150/ijms.66996 This issue Cite

Research Paper

Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats

Tzu-Jung Chou1,2, Chia-Wen Lu1,2,3, Chen-Chung Liao4, Chien-Hsieh Chiang1,3,5, Chi-Chang Huang6✉, Kuo-Chin Huang1,3,7✉

1. Department of Family Medicine, National Taiwan University Hospital, Taipei, Taiwan.
2. Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
3. Department of Family Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
4. Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan.
5. Graduate Institute of Pharmacology, National Taiwan University College of Medicine, Taipei, Taiwan.
6. Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan, Taiwan.
7. Department of Family Medicine, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan.

Citation:
Chou TJ, Lu CW, Liao CC, Chiang CH, Huang CC, Huang KC. Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats. Int J Med Sci 2022; 19(3):499-510. doi:10.7150/ijms.66996. https://www.medsci.org/v19p0499.htm
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Abstract

Graphic abstract

Postmenopausal women exhibit a higher prevalence of obesity due to decreased energy expenditure and increased food intake compared to their premenopausal counterparts. Brown adipose tissue (BAT) plays a key role in energy homeostasis, thus providing us with appealing therapeutic targets in obesity. However, how BAT proteomes are altered in response to low levels of estrogen remains unclear. To better understand the underlying mechanisms between the postmenopausal state and BAT proteomic changes, our study aimed to investigate the effect of ovariectomy on the BAT proteome. In this study, eight-week-old female Sprague Dawley rats were randomly allocated into bilateral ovariectomy (Ovx) and sham operation (Sham) groups. Mass spectrometry was used for proteomics assay and Ingenuity Pathway Analysis was applied to examine the differentially regulated proteins. Of the 1,412 identified proteins, 18 proteins were significantly upregulated, whereas 36 proteins were significantly downregulated in the Ovx group as compared to the Sham group. Our findings demonstrate that the proteins involved in BAT morphology, the browning of white adipose tissue, and metabolic substrates for thermogenesis were regulated by ovariectomy. The dysregulation of proteins by ovariectomy might be related to the disruption of BAT function in the postmenopausal status. Understanding how BAT proteomes are altered in response to ovariectomy may illuminate novel therapeutic strategies for the management of postmenopausal weight gain in the future.

Keywords: brown adipose tissue, ingenuity pathways analysis, menopause, obesity, proteome


Citation styles

APA
Chou, T.J., Lu, C.W., Liao, C.C., Chiang, C.H., Huang, C.C., Huang, K.C. (2022). Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats. International Journal of Medical Sciences, 19(3), 499-510. https://doi.org/10.7150/ijms.66996.

ACS
Chou, T.J.; Lu, C.W.; Liao, C.C.; Chiang, C.H.; Huang, C.C.; Huang, K.C. Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats. Int. J. Med. Sci. 2022, 19 (3), 499-510. DOI: 10.7150/ijms.66996.

NLM
Chou TJ, Lu CW, Liao CC, Chiang CH, Huang CC, Huang KC. Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats. Int J Med Sci 2022; 19(3):499-510. doi:10.7150/ijms.66996. https://www.medsci.org/v19p0499.htm

CSE
Chou TJ, Lu CW, Liao CC, Chiang CH, Huang CC, Huang KC. 2022. Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats. Int J Med Sci. 19(3):499-510.

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