Int J Med Sci 2020; 17(8):1056-1061. doi:10.7150/ijms.43981 This issue Cite

Research Paper

Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1

Hua Liu1✉, Yinping Li1, Na Lin1, Xingtong Dong1, Wen Li1, Yinghui Deng1, Lina Ma2

1. Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
2. Department of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Citation:
Liu H, Li Y, Lin N, Dong X, Li W, Deng Y, Ma L. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int J Med Sci 2020; 17(8):1056-1061. doi:10.7150/ijms.43981. https://www.medsci.org/v17p1056.htm
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Abstract

The aim of this study was to determine whether interleukin-1β (IL-1β) promotes oxidised low-density lipoprotein (Ox-LDL) uptake by human glomerular mesangial cells (HMCs) and its effect on the expression of lectin-like Ox-LDL receptor 1 (LOX-1) and to identify pathways through which IL-1β affects lipid uptake. Confocal laser scanning microscopy and flow cytometry were used to observe the effect of IL-1β on lipid uptake by HMCs and the pathway by which IL-1β may mediate lipid uptake. Real-time polymerase chain reaction (PCR) and western blotting were used to evaluate the effect of IL-1β on LOX-1 expression. Confocal laser scanning microscopy and flow cytometry revealed that IL-1β promoted uptake of fluorescent Dil-labelled Ox-LDL(Dil-Ox-LDL) by HMCs and the enhanced uptake of Dil-Ox-LDL was partially inhibited by an anti-LOX-1 antibody evaluated by flow cytometry. Further, IL-1β promoted LOX-1 mRNA and protein expression of HMCs in a dose- and time-dependent manner. Thus, Ox-LDL is ingested by HMCs under basic conditions. Inflammatory cytokine IL-1β promotes Ox-LDL uptake by HMCs. Furthermore, IL-1β promotes the mRNA and protein expression of LOX-1, a specific receptor of Ox-LDL, suggesting that the enhancement of Ox-LDL uptake may be mediated by LOX-1 pathway. Anti-LOX-1 therapy may be a promising option for treatment of glomerulosclerosis.

Keywords: Interleukin-1β, oxidised low-density lipoprotein, lectin-like Ox-LDL receptor 1


Citation styles

APA
Liu, H., Li, Y., Lin, N., Dong, X., Li, W., Deng, Y., Ma, L. (2020). Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. International Journal of Medical Sciences, 17(8), 1056-1061. https://doi.org/10.7150/ijms.43981.

ACS
Liu, H.; Li, Y.; Lin, N.; Dong, X.; Li, W.; Deng, Y.; Ma, L. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int. J. Med. Sci. 2020, 17 (8), 1056-1061. DOI: 10.7150/ijms.43981.

NLM
Liu H, Li Y, Lin N, Dong X, Li W, Deng Y, Ma L. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int J Med Sci 2020; 17(8):1056-1061. doi:10.7150/ijms.43981. https://www.medsci.org/v17p1056.htm

CSE
Liu H, Li Y, Lin N, Dong X, Li W, Deng Y, Ma L. 2020. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int J Med Sci. 17(8):1056-1061.

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