Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1

Liu, Hua; Li, Yinping; Lin, Na; Dong, Xingtong; Li, Wen; Deng, Yinghui; Ma, Lina

doi:10.7150/ijms.43981

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Int J Med Sci 2020; 17(8):1056-1061. doi:10.7150/ijms.43981 This issue Cite

Research Paper

Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1

Hua Liu^1✉, Yinping Li¹, Na Lin¹, Xingtong Dong¹, Wen Li¹, Yinghui Deng¹, Lina Ma²

1. Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
2. Department of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Citation:

Liu H, Li Y, Lin N, Dong X, Li W, Deng Y, Ma L. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int J Med Sci 2020; 17(8):1056-1061. doi:10.7150/ijms.43981. https://www.medsci.org/v17p1056.htm

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Abstract

The aim of this study was to determine whether interleukin-1β (IL-1β) promotes oxidised low-density lipoprotein (Ox-LDL) uptake by human glomerular mesangial cells (HMCs) and its effect on the expression of lectin-like Ox-LDL receptor 1 (LOX-1) and to identify pathways through which IL-1β affects lipid uptake. Confocal laser scanning microscopy and flow cytometry were used to observe the effect of IL-1β on lipid uptake by HMCs and the pathway by which IL-1β may mediate lipid uptake. Real-time polymerase chain reaction (PCR) and western blotting were used to evaluate the effect of IL-1β on LOX-1 expression. Confocal laser scanning microscopy and flow cytometry revealed that IL-1β promoted uptake of fluorescent Dil-labelled Ox-LDL(Dil-Ox-LDL) by HMCs and the enhanced uptake of Dil-Ox-LDL was partially inhibited by an anti-LOX-1 antibody evaluated by flow cytometry. Further, IL-1β promoted LOX-1 mRNA and protein expression of HMCs in a dose- and time-dependent manner. Thus, Ox-LDL is ingested by HMCs under basic conditions. Inflammatory cytokine IL-1β promotes Ox-LDL uptake by HMCs. Furthermore, IL-1β promotes the mRNA and protein expression of LOX-1, a specific receptor of Ox-LDL, suggesting that the enhancement of Ox-LDL uptake may be mediated by LOX-1 pathway. Anti-LOX-1 therapy may be a promising option for treatment of glomerulosclerosis.

Keywords: Interleukin-1β, oxidised low-density lipoprotein, lectin-like Ox-LDL receptor 1

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APA

Liu, H., Li, Y., Lin, N., Dong, X., Li, W., Deng, Y., Ma, L. (2020). Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. International Journal of Medical Sciences, 17(8), 1056-1061. https://doi.org/10.7150/ijms.43981.

ACS

Liu, H.; Li, Y.; Lin, N.; Dong, X.; Li, W.; Deng, Y.; Ma, L. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int. J. Med. Sci. 2020, 17 (8), 1056-1061. DOI: 10.7150/ijms.43981.

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CSE

Liu H, Li Y, Lin N, Dong X, Li W, Deng Y, Ma L. 2020. Interleukin-1β Promotes Ox-LDL Uptake by Human Glomerular Mesangial Cells via LOX-1. Int J Med Sci. 17(8):1056-1061.

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