Int J Med Sci 2020; 17(5):640-646. doi:10.7150/ijms.39631 This issue

Research Paper

Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study

Hyun Jin Min1, Sung Jin Min1, Hyun Kang2, Kyung Soo Kim1✉

1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Chung- Ang University Hospital, Seoul, Republic of Korea.
2. Department of Anesthesiology and Pain Medicine, College of Medicine, Chung- Ang University Hospital, Seoul, Republic of Korea.

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Citation:
Min HJ, Min SJ, Kang H, Kim KS. Differential Nasal Expression of Heat Shock Proteins 27 and 70 by Aerobic Exercise: A Preliminary Study. Int J Med Sci 2020; 17(5):640-646. doi:10.7150/ijms.39631. Available from https://www.medsci.org/v17p0640.htm

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Abstract

Purpose: Exercise modifies airway immune responses and susceptibility to infection. We investigated the effects of exercise on two HSPs (heat shock proteins), quantifying expression levels in nasal mucosa of both professional competitive athletes and non-athletes for comparison.

Method: We used western blot technique to determine expression levels of HSPs in primary human nasal epithelial cells (HNECs). Nasal lavage (NAL) fluids were collected from 12 male professional volley ball players and 6 healthy males pre-submaximal exercise (running for 30 min at 70-80% of maximal heart rate) and post-submaximal exercise. Expression levels of HSP27, HSP70, Interleukin (IL)-8, and Tumor necrosis factor (TNF)-α in NAL fluids were quantified by enzyme-linked immunosorbent assay (ELISA), and difference of the level between pre-submaximal exercise and post-submaximal exercise was statistically analyzed. Antibacterial assay using Staphylococcus aureus was performed to assess the immunological role of HSPs in NAL fluids.

Results:. In non-athlete controls, HSP27, HSP70, and IL-8 were unchanged after exercise. In the professional athletes, HSP70 expression was declined significantly (p<0.05), but HSP27 was not significantly changed. IL-8 and TNF-α did not show significant difference, either. By antibacterial assay, it was found that the number of active bacterial populations were influenced by the presence or absence of HSP27 and HSP70 in NAL fluids.

Conclusion: HSP27 and HSP70 were present in NAL fluids of enrolled subjects, and the effect of exercise on the level HSPs was different between professional athletic competitors and non-athletes. As the number of active bacterial population was influenced by the presence or absence of nasal HSP27 and HSP70, we suggest that HSP27 and HSP70 may play immunological function in NAL fluids.

Keywords: Hsp70, Hsp27, nasal mucosa, exercise, athletes