1. Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
2. Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
3. Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
4. Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan
5. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan
Hyperpigmentation is a type of pigmentary disorder induced by overexpression of melanin content activated severe esthetic problems as melasma, freckle, ephelides, lentigo and other forms on human skin. Several whitening agents have restricted use because of their side effects or stability such as kojic acid, ascorbic acid and hydroquinone can act as cytotoxic substance which associated to dermatitis and skin cancer. To find for the safe substance, this study aimed to find for the ability of several components in Sucrier banana peel (SBP) extracts to inhibit melanogenesis process through p38 signaling pathway in B16F10 mouse melanoma cells. Tyrosinase activity and the cellular melanin content were dose dependent manner decreasing after SBP treatment. Furthermore, SBP decreased the expression of melanogenesis relate protein as microphthalmia-associated transcription factor (MITF) and tyrosinase protein after 24 hours incubation with α-melanocyte stimulating hormones (MSH) stimulating. The findings demonstrated that SBP contained an effective agent for hyperpigmentation inhibitor through p38 signaling pathways without any effect to ERK pathway, and subsequent down-regulate MITF expression and tyrosinase enzyme family production.
Keywords: sucrier banana peel, phenolic compounds, tyrosinase, microphthalmia-associated transcription factor, melanogenesis