Int J Med Sci 2014; 11(3):222-225. doi:10.7150/ijms.7382 This issue
Short Research Communication
1. Department of Pharmacology, Kagawa University Medical School, Kagawa, Japan.
2. Teijin Institute for Bio-Medical Research, Teijin Pharma Ltd., Tokyo, Japan.
3. Department of Gastroenterology and Neurology, Kagawa University Medical School, Kagawa, Japan.
4. Department of Cardiovascular Diseases, Fukuoka University Chikushi Hospital, Fukuoka, Japan.
We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.
Keywords: human chymase transgenic mice, chymase activity and lipopolysaccharide, endotoxemia