Int J Med Sci 2022; 19(2):242-256. doi:10.7150/ijms.67741 This issue

Research Paper

Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy

Jai-Hong Cheng1,2,3, Wen-Yi Chou1,4, Ching-Jen Wang1,4, Ka-Kit Siu1,4,5, Jei-Ming Peng6, Yi-No Wu7, Meng-Shiou Lee8, Chien-Yiu Huang1, Jih-Yang Ko1,4✉, Shun-Wun Jhan1,4✉

1. Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
2. Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
3. Department of Leisure and Sports Management, Cheng Shiu University, Kaohsiung, Taiwan.
4. Department of Orthopedic Surgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
5. Park One International Hospital, Kaohsiung, Taiwan.
6. Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
7. School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
8. Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, 91, Hsueh-Shih Road, Taichung, Taiwan.

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Citation:
Cheng JH, Chou WY, Wang CJ, Siu KK, Peng JM, Wu YN, Lee MS, Huang CY, Ko JY, Jhan SW. Pathological, Morphometric and Correlation Analysis of the Modified Mankin Score, Tidemark Roughness and Calcified Cartilage Thickness in Rat Knee Osteoarthritis after Extracorporeal Shockwave Therapy. Int J Med Sci 2022; 19(2):242-256. doi:10.7150/ijms.67741. Available from https://www.medsci.org/v19p0242.htm

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Abstract

Graphic abstract

The paper displayed the pathological changes and relationships of the modified Mankin score, tidemark roughness and calcified cartilage (CC) thickness by extracorporeal shockwave therapy (ESWT) (0.25 mJ/ mm2 with 800 impulses) on different positions of the medial and lateral rat knee OA joint. After the experiments, the articular cartilage was assessed using histomorphometry, image analysis and statistical method. In the micro-CT analysis, ESWT on medial groups were better than lateral groups in the trabecular volume and trabecular number. The data showed a strong negative correlation between the modified Mankin score and tidemark roughness (r = -0.941; P < 0.001). In terms of the relationship of tidemark roughness with CC thickness, the medial and Sham groups showed a significant negative correlation (r = -0.788, P = 0.022). Additionally, the Euclidean distance derived from 3D scatter plot analysis was an indicator of chondropathic conditions, exhibiting a strong correlation with OA stage in the articular cartilage of the femur (r = 0.911, P < 0.001) and tibia (r = 0.890, P < 0.001) after ESWT. Principle component analysis (PCA) further demonstrated that ESWT applied to medial locations had a better outcome than treatment at lateral locations for knee OA by comparing with Sham and OA groups, and CC thickness was the most important factor affecting hyaline cartilage repair after ESWT.

Keywords: osteoarthritis, extracorporeal shockwave therapy, tidemark roughness, calcified cartilage thickness, statistical analysis.