Int J Med Sci 2020; 17(8):1006-1014. doi:10.7150/ijms.39003 This issue Cite

Research Paper

Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells

Ji-Yeon Park1, Sung-Gang Jo1, Ha-Nul Lee2, Joo-Hee Choi1,3, Yeon-Ji Lee1, Young-Min Kim2, Jeong-Yong Cho2, Sung Ki Lee4, Jong-Hwan Park1,✉

1. Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju, Republic of Korea
2. Department of Food Science & Technology, Chonnam National University, Gwangju, Republic of Korea
3. Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea
4. Department of Obstetrics and Gynecology, College of Medicine, Konyang University, Daejeon, Republic of Korea

Citation:
Park JY, Jo SG, Lee HN, Choi JH, Lee YJ, Kim YM, Cho JY, Lee SK, Park JH. Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. Int J Med Sci 2020; 17(8):1006-1014. doi:10.7150/ijms.39003. https://www.medsci.org/v17p1006.htm
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Abstract

Inflammation is the root cause of many diseases that pose a serious threat to human health. Excessive inflammation can also result in preterm birth or miscarriage in pregnant women. Pumpkin (Cucurbita moschata Duchesne, CMD) is a well-known traditional health food and medicinal herb used in many countries to treat diabetes, obesity, osteoporosis, cancer and other diseases. In this study, we investigated the effects of hot water extract derived from the tendrils of C. moschata Duchesne (TCMD) on NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. The TCMD treatment of LPS-primed bone marrow-derived macrophages (BMDMs) and human trophoblast cells attenuated NLRP3 inflammasome activation induced by inflammasome activators such as ATP, nigericin, and monosodium urate (MSU). TCMD treatment suppressed IL-1β secretion in a dose-dependent manner, without affecting IL-6 secretion. In addition, TCMD inhibited NLRP3-dependent pyroptosis in BMDMs. TCMD also suppressed the release of mature IL-1β and activation of cleaved-caspase-1 via limited ASC oligomerization. Furthermore, TCMD significantly inhibited IL-1β secretion and pyroptotic cell death in human trophoblast cells. These results suggest that TCMD exhibits anti-inflammatory effects mediated via inhibition of NLRP3 inflammasome activation suggesting therapeutic potential against inflammatory diseases, preterm birth, and miscarriage.

Keywords: tendril of Cucurbita moschata Duch, NLRP3 inflammasome, bone marrow derived macrophage, trophoblast, SW.71


Citation styles

APA
Park, J.Y., Jo, S.G., Lee, H.N., Choi, J.H., Lee, Y.J., Kim, Y.M., Cho, J.Y., Lee, S.K., Park, J.H. (2020). Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. International Journal of Medical Sciences, 17(8), 1006-1014. https://doi.org/10.7150/ijms.39003.

ACS
Park, J.Y.; Jo, S.G.; Lee, H.N.; Choi, J.H.; Lee, Y.J.; Kim, Y.M.; Cho, J.Y.; Lee, S.K.; Park, J.H. Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. Int. J. Med. Sci. 2020, 17 (8), 1006-1014. DOI: 10.7150/ijms.39003.

NLM
Park JY, Jo SG, Lee HN, Choi JH, Lee YJ, Kim YM, Cho JY, Lee SK, Park JH. Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. Int J Med Sci 2020; 17(8):1006-1014. doi:10.7150/ijms.39003. https://www.medsci.org/v17p1006.htm

CSE
Park JY, Jo SG, Lee HN, Choi JH, Lee YJ, Kim YM, Cho JY, Lee SK, Park JH. 2020. Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in murine macrophages and human trophoblast cells. Int J Med Sci. 17(8):1006-1014.

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