Int J Med Sci 2020; 17(7):931-938. doi:10.7150/ijms.43030 This issue Cite

Research Paper

Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population

Shuying Dai2*, Chuanyin Li1*, Zhiling Yan3, Ziyun Zhou1, Xia Wang3, Jun Wang3, Le Sun2, Li Shi1✉, Yufeng Yao1✉

1. Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China.
2. School of Basic Medical Science, Kunming Medical University, Kunming 650500, China.
3. Department of Gynaecologic Oncology, The 3rd Affiliated Hospital of Kunming Medical University, Kunming 650118, China.
*These authors contributed equally to this work.

Citation:
Dai S, Li C, Yan Z, Zhou Z, Wang X, Wang J, Sun L, Shi L, Yao Y. Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population. Int J Med Sci 2020; 17(7):931-938. doi:10.7150/ijms.43030. https://www.medsci.org/v17p0931.htm
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Abstract

Objective: High-risk human papillomavirus (HPV) E6 and E7 proteins are the major oncoproteins involved in the tumorigenesis of cervical cancer. The long control region (LCR) in HPV plays an important role in regulating the expression of the E6 and E7 oncogenes. In the current study, we investigated the association of HPV16 LCR variations with cervical cancer.

Methods: A total of 139 HPV16-positive cervical cancer patients (case group) and 116 HPV16-positive asymptomatic individuals (control group) were enrolled in the current study. Then, the HPV16 LCR was sequenced to determine the association between LCR variations and cervical cancer.

Results: In the current study, HPV16 A1-A3 (19.4%), A4 (78.4%) and D3 (2.2%) variants were found in the case group. However, only A1-A3 (34.5%) and A4 variants (65.5%) were found in the control group. The distribution of the HPV16 variants between the case and control groups was significantly different (P=0.009). Moreover, a total of eleven variations (A7167G, A7173C, C7176T, C7200T, T7269C, C7286A, C7729A, C7763T, A7841G, G7867A and T24C) were significantly different between the case and control groups (P<0.05). For the sub-lineage analysis, only C7873G variations were significantly different between the case and control groups in the A4 (As) variant (P=0.039).

Conclusion: Our results showed that specific variations in the HPV16 LCR were associated with cervical cancer. Our study will provide a good reference for further understanding of the relationship between HPV16 LCR variation and cervical cancer.

Keywords: Human papillomavirus type 16, LCR, Variations, Cervical cancer, Association


Citation styles

APA
Dai, S., Li, C., Yan, Z., Zhou, Z., Wang, X., Wang, J., Sun, L., Shi, L., Yao, Y. (2020). Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population. International Journal of Medical Sciences, 17(7), 931-938. https://doi.org/10.7150/ijms.43030.

ACS
Dai, S.; Li, C.; Yan, Z.; Zhou, Z.; Wang, X.; Wang, J.; Sun, L.; Shi, L.; Yao, Y. Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population. Int. J. Med. Sci. 2020, 17 (7), 931-938. DOI: 10.7150/ijms.43030.

NLM
Dai S, Li C, Yan Z, Zhou Z, Wang X, Wang J, Sun L, Shi L, Yao Y. Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population. Int J Med Sci 2020; 17(7):931-938. doi:10.7150/ijms.43030. https://www.medsci.org/v17p0931.htm

CSE
Dai S, Li C, Yan Z, Zhou Z, Wang X, Wang J, Sun L, Shi L, Yao Y. 2020. Association of Human Papillomavirus Type 16 Long Control Region Variations with Cervical Cancer in a Han Chinese Population. Int J Med Sci. 17(7):931-938.

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