1. Laboratory of Pathogenic Biology, Guangdong Medical University, Zhanjiang 524023, China;
2. Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan 523808, China;
3. Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China;
4. Department of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
5. Department of Blood Internal Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China;
6. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China;
7. School of Chinese Medicine, The University of Hongkong, Pokfulam, Hongkong;
8. Department of Cardiothoracic Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
Yunnan Baiyao (YB) as a kind of famous Chinese herbal medicine, possessed hemostatic, invigorating the circulation of blood, and anti-inflammatory effects. Identifying strategies to protect patients at risk for hospital-acquired pressure ulcers (HAPU) is essential. Herein, our results showed that YB treatment can effectively reduce the acne wound area and improve efficacy in a comparative study of 60 cases HAPU patients with S. aureus positive of acne wound pathogens. Furthermore, YB inhibited HIa expression and suppressed accessory gene regulator (agr) system controlled by regulatory RNA II and RNA III molecule using pALC1740, pALC1742 and pALC1743 S. aureus strain linked to gfpuvr reporter gene. Moreover, YB downregulated cao mRNA expression and inhibited coagulase activity by RT-PCR, slide and tube coagulase test. Additionally, YB downregulated seb, sec, sed, and tsst-1 mRNA expression to suppress enterotoxin and tsst-1 secretion and adhesion function related genes sarA, icaA, and cidA mRNA expression. Taken together, the data suggest that YB may reduce HAPU via suppressing virulence gene expression and biofilm formation of S. aureus.
Keywords: Yannan Baiyao, hospital-acquired pressure ulcers, Staphylococcus aureus, agr system, virulence factors, biofilms