1. Graduate Faculty, Jiangxi Medical College, Nanchang University, Nanchang 330006;
2. Department of Urology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang 330006;
3. Departments of Gerontology, The First Affiliated Hospital of Nanchang University, Nanchang 330006;
4. Division of Nephrology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240;
5. Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang 330006;
6. Department of Respiratory Medicine, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang 330006.
Bladder cancer is a common malignant urinary tumor, and patients with bladder cancer have poor prognosis. Abnormal lipid metabolism in peroxisomes is involved in tumor progression. Hydroxysteroid dehydrogenase-like 2 (HSDL2) localized in peroxisomes regulates fatty acid synthesis. In the present study, we reported that HSDL2 was upregulated in two human bladder cancer cell lines 5637 and T24 compared to normal human urothelial cells. Furthermore, lentiviral-mediated HSDL2 knockdown inhibited the proliferation and colony formation while promoted the apoptosis of human bladder cancer T24 cells in vitro. In nude mice HSDL2 knockdown inhibited the growth of T24 derived xenografts in vivo. In conclusion, our results suggest that HSDL2 plays an oncogenic role in bladder cancer and might serve as a potential target for bladder cancer therapy.
Keywords: bladder cancer, HSDL2, shRNA, cell proliferation, apoptosis