Knockdown of <i>NPM1</i> by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line

Qin, Feng-Xian; Shao, Hui-Yuan; Chen, Xian-Chun; Tan, Shi; Zhang, Hui-Juan; Miao, Zong-Yu; Wang, Li; Hui-Chen,; Zhang, Ling

doi:10.7150/ijms.8.287



Theranostics

International Journal of Biological Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Med Sci 2011; 8(4):287-294. doi:10.7150/ijms.8.287 This issue Cite

Research Paper

Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line

Feng-Xian Qin¹, Hui-Yuan Shao¹, Xian-Chun Chen¹, Shi Tan¹, Hui-Juan Zhang¹, Zong-Yu Miao¹, Li Wang², Hui-Chen³, Ling Zhang^{1 ✉}

1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
2. Department of Hematology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
3. Department of Laboratory Medicine, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.

Citation:

Qin FX, Shao HY, Chen XC, Tan S, Zhang HJ, Miao ZY, Wang L, Hui-Chen , Zhang L. Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line. Int J Med Sci 2011; 8(4):287-294. doi:10.7150/ijms.8.287. https://www.medsci.org/v08p0287.htm

Other styles

Abstract

Nucleophosmin (NPM1) is an abundant and ubiquitously expressed phosphoprotein that is known to influence solid tumors progression. However, little is known about the role of NPM1 in leukemia. Here, we knocked down the NPM1 expression by RNA interference to investigate the role of NPM1 in leukemic cells proliferation and apoptosis. The interference vector pNPM1-shRNA was constructed and transfected into the human leukemic K562 cell line. The expression levels of NPM1 mRNA and protein were detected by quantitative real-time PCR and Western blot, respectively. Cells proliferation potential in vitro was assessed by methyl thiazolyl tetrazolium (MTT) and colony formation assays. Flow cytometry was used to detect the distribution of cell cycle. Cellular apoptosis was reflected by the relative activities of caspase-3 and caspase-8. The results showed that the expression levels of NPM1 mRNA and protein in K562 cells were significantly reduced after pNPM1-shRNA transfection. The cells growth was significantly inhibited in a time-dependent manner and the number of colonies was significantly reduced in the pNPM1-shRNA transfected cells. Meanwhile, the percentage of cells in G1 phase in the K562/pNPM1-shRNA cells was significantly increased. In addition, there were higher relative activities of caspase-3/8 in the pNPM1-shRNA transfected cells. These results indicate that down-regulation of NPM1 expression inhibits leukemic cells proliferation, blocks cell cycle progression and induces cellular apoptosis. It may implicate a potential target for leukemia gene therapy.

Keywords: Nucleophosmin, RNA interference, leukemic cell, proliferation, apoptosis

Citation styles

APA

Qin, F.X., Shao, H.Y., Chen, X.C., Tan, S., Zhang, H.J., Miao, Z.Y., Wang, L., Hui-Chen, , Zhang, L. (2011). Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line. International Journal of Medical Sciences, 8(4), 287-294. https://doi.org/10.7150/ijms.8.287.

ACS

Qin, F.X.; Shao, H.Y.; Chen, X.C.; Tan, S.; Zhang, H.J.; Miao, Z.Y.; Wang, L.; Hui-Chen, ; Zhang, L. Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line. Int. J. Med. Sci. 2011, 8 (4), 287-294. DOI: 10.7150/ijms.8.287.

NLM

CSE

Qin FX, Shao HY, Chen XC, Tan S, Zhang HJ, Miao ZY, Wang L, Hui-Chen , Zhang L. 2011. Knockdown of NPM1 by RNA Interference Inhibits Cells Proliferation and Induces Apoptosis in Leukemic Cell Line. Int J Med Sci. 8(4):287-294.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.