Int J Med Sci 2006; 3(3):97-105. doi:10.7150/ijms.3.97 This issue Cite

Research Paper

Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors

Hongwei Li1, Jin Zhong Li1, Debra D. Pittman2, Andy Amalfitano3, Gerald R. Hankins1, Gregory A. Helm1 4

1 Departments of Neurological Surgery, University of Virginia Health System, Charlottesville, Virginia 22908, USA
2 Genetics Institute, Andover, Massachusetts 01810, USA
3 Departments of Pediatrics and Human Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA
4 Departments of Biomedical Engineering, University of Virginia Health System, Charlottesville, Virginia 22908, USA

Citation:
Li H, Li JZ, D. Pittman D, Amalfitano A, Hankins GR, Helm GA. Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. Int J Med Sci 2006; 3(3):97-105. doi:10.7150/ijms.3.97. https://www.medsci.org/v03p0097.htm
Other styles

File import instruction

Abstract

Osteogenic potentials of some recombinant human bone morphogenetic protein (BMP) first-generation adenoviral vectors (ADhBMPs) are significantly limited in immunocompetent animals. It is unclear what role expression of viral proteins and foreign proteins transduced by adenoviral vectors play in the host immune response and in ectopic bone formation. In this study two sets of experiments were designed and performed. First, rat BMP6 cDNA were amplified, sequenced, and recombined in first-generation adenoviral vector (ADrBMP6). A comparison of human and rat BMP6 adenoviral vectors demonstrated identical osteogenic activities in both immunodeficient and immunocompetent rats. Second, the activities of recombinant human BMP6 in E1- (ADhBMP6) and [E1-,E2b-] ( [E1-,E2b-]ADGFP&hBMP6, and [E1-,E2b-]ADhBMP6) adenoviral vectors were compared in both in vitro and in vivo models. Similar activities of these two generations of BMP adenoviral vectors were found in all models. These results indicate that the amount of viral gene expression and the source of the BMP cDNA are not major factors in the interruption of osteogenic potentials of recombinant BMP6 adenoviral vectors in immunocompetent animals.


Citation styles

APA
Li, H., Li, J.Z., D. Pittman, D., Amalfitano, A., Hankins, G.R., Helm, G.A. (2006). Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. International Journal of Medical Sciences, 3(3), 97-105. https://doi.org/10.7150/ijms.3.97.

ACS
Li, H.; Li, J.Z.; D. Pittman, D.; Amalfitano, A.; Hankins, G.R.; Helm, G.A. Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. Int. J. Med. Sci. 2006, 3 (3), 97-105. DOI: 10.7150/ijms.3.97.

NLM
Li H, Li JZ, D. Pittman D, Amalfitano A, Hankins GR, Helm GA. Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. Int J Med Sci 2006; 3(3):97-105. doi:10.7150/ijms.3.97. https://www.medsci.org/v03p0097.htm

CSE
Li H, Li JZ, D. Pittman D, Amalfitano A, Hankins GR, Helm GA. 2006. Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. Int J Med Sci. 3(3):97-105.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Popup Image