Int J Med Sci 2020; 17(5):626-631. doi:10.7150/ijms.36484 This issue Cite

Research Paper

Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation

Minho Kim1, JaeGoo Kim1, Yu-Kyong Shin2, Ki-Young Kim1,2✉

1. Graduate School of Biotechnology, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea
2. College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea

Citation:
Kim M, Kim J, Shin YK, Kim KY. Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation. Int J Med Sci 2020; 17(5):626-631. doi:10.7150/ijms.36484. https://www.medsci.org/v17p0626.htm
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Abstract

Keratinocyte proliferation is important for skin wound healing. The wound healing process includes blood clotting around the wound, removal of dead cells and pathogens through inflammation, and then re-epithelialization through proliferation and maturation. Proliferation assay was performed on acid natural compounds to identify candidates for natural-derived components of skin injury treatment. We found that gentisic acid promoted high cell proliferation activity compared with other compounds. Gentisic acid improved HaCaT cell proliferation by over 20% in MTT assay. Gentisic acid also had higher healing activity in an in vitro wound healing assay than allantoin as a positive control. Furthermore, we have identified how the treatment of gentisic acid can increase proliferation in the cell. Western blot analysis of proteins in the mitogen-activated protein (MAP) kinase signaling pathway showed that ERK1/2 phosphorylation was increased by gentisic acid treatment. Thus, our study indicates that gentisic acid promotes the proliferation of keratinocyte by phosphorylation of ERK1/2.

Keywords: HaCaT cell, MAP kinase pathway, wound healing, re-epithelialization


Citation styles

APA
Kim, M., Kim, J., Shin, Y.K., Kim, K.Y. (2020). Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation. International Journal of Medical Sciences, 17(5), 626-631. https://doi.org/10.7150/ijms.36484.

ACS
Kim, M.; Kim, J.; Shin, Y.K.; Kim, K.Y. Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation. Int. J. Med. Sci. 2020, 17 (5), 626-631. DOI: 10.7150/ijms.36484.

NLM
Kim M, Kim J, Shin YK, Kim KY. Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation. Int J Med Sci 2020; 17(5):626-631. doi:10.7150/ijms.36484. https://www.medsci.org/v17p0626.htm

CSE
Kim M, Kim J, Shin YK, Kim KY. 2020. Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation. Int J Med Sci. 17(5):626-631.

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