Int J Med Sci 2019; 16(10):1320-1327. doi:10.7150/ijms.33728 This issue

Review

Distinct gene expression characteristics in epithelial cell-Porphyromonas gingivalis interactions by integrating transcriptome analyses

Dongmei Zhang1, Jingya Hou2, Yun Wu2, Yanqing Liu2, Rong Li2, Tong Xu2, Junchao Liu1, Yaping Pan1✉

1. Department of Periodontics and Oral Biology, School of Stomatology, China Medical University, Shenyang 110002, China
2. Department of Periodontics, School of Stomatology, China Medical University, Shenyang 110002, China

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Citation:
Zhang D, Hou J, Wu Y, Liu Y, Li R, Xu T, Liu J, Pan Y. Distinct gene expression characteristics in epithelial cell-Porphyromonas gingivalis interactions by integrating transcriptome analyses. Int J Med Sci 2019; 16(10):1320-1327. doi:10.7150/ijms.33728. Available from https://www.medsci.org/v16p1320.htm

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Abstract

Porphyromonas gingivalis is a pivotal periodontal pathogen, and the epithelial cells serve as the first physical barrier to defend the host from bacterial attack. Within this host-bacteria interaction, P. gingivalis can modify the host immune reaction and adjust the gene expression, which is associated with periodontitis pathogenesis and developing strategies. Herein, a meta-analysis was made to get the differential gene expression profiles in epithelial cells with or without P. gingivalis infection. The network-based meta-analysis program for gene expression profiling was used. Both the gene ontology analysis and the pathway enrichment analysis of the differentially expressed genes were conducted. Our results determined that 290 genes were consistently up-regulated in P. gingivalis infected epithelial cells. 229 gene ontology biological process terms of up-regulated genes were discovered, including “negative regulation of apoptotic process” and “positive regulation of cell proliferation/migration/angiogenesis”. In addition to the well-known inflammatory signaling pathways, the pathway associated with a transcriptional misregulation in cancer has also been increased. Our findings indicated that P. gingivalis benefited from the survival of epithelial cells, and got its success as a colonizer in oral epithelium. The results also suggested that infection of P. gingivalis might contribute to oral cancer through chronic inflammation. Negative regulation of the apoptotic process and transcriptional misregulation in cancer pathway are important contributors to the cellular physiology changes during infection development, which have particular relevance to the pathogenesis and progressions of periodontitis, even to the occurrence of oral cancer.

Keywords: Differentially expressed gene, Epithelial cell, Meta-analysis, Microarray, Periodontitis, Porphyromonas gingivalis