Int J Med Sci 2015; 12(10):759-763. doi:10.7150/ijms.12882 This issue

Research Paper

Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas

Keisuke Takamine1, Yukiko Ueda1, Keisuke Nakano1, 2, Takanaga Ochiai1, Yoshihiko Sugita3, Katsutoshi Kubo3, Hatsuhiko Maeda3, Hiromasa Hasegawa1, Toshiyuki Kawakami1✉

1. Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan
2. Department of Oral Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmacuitical Sciences, Okayama, Japan
3. Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan

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Takamine K, Ueda Y, Nakano K, Ochiai T, Sugita Y, Kubo K, Maeda H, Hasegawa H, Kawakami T. Notch as a Possible Cell Differentiation Factor in Pleomorphic Adenomas. Int J Med Sci 2015; 12(10):759-763. doi:10.7150/ijms.12882. Available from

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The expression of Notch in 30 cases of pleomorphic adenoma was examined by immunohistochemistry. Comparing the results of our study with previous literatures, from the partial CK7 expression and substantial Notch expression in ductal epithelial cells as well as the Notch expression in solid tumor nests, it can be inferred that Notch is involved in cell differentiation. CK13 expression was observed in cells undergoing squamous metaplasia and Notch expression was seen in the nucleus of basal and squamous cells. The intense Notch expression in basal cells and weak expression in squamous cells suggests that Notch is involved in the differentiation from basal to squamous cell. Moreover, the loss of nuclear expression on the inner layer would signify that differentiation is about to end or has been terminated. Notch was expressed in the cytoplasm of cartilage cells and in the cell membrane of mucous cells but not in the nucleus indicating that differentiation has been concluded. Notch involvement is suspected in cell differentiation in areas showing ductal structures and squamous metaplasia. In summary, Notch is involved in cell differentiation of ductal cells in PA. Nuclear expression was shown in tumor cells in solid nests and surrounding structures. Moreover, Notch is expressed by basal cells undergoing squamous metaplasia suggesting the participation of Notch in cell differentiation in PA.

Keywords: Notch1, pleomorphic adenoma, cell differentiation, immunohistochemistry