Int J Med Sci 2023; 20(4):530-541. doi:10.7150/ijms.80498 This issue Cite

Research Paper

Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19

Marina Jovanovic1, Sofija Sekulic2, Miodrag Jocic3✉, Milena Jurisevic4, Nevena Gajovic5, Marina Jovanovic6, Nebojsa Arsenijevic5, Milan Jovanovic7, Milan Mijailovic8, Milos Milosavljevic5, Ivan Jovanovic5

1. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Otorhinolaringology
2. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Infectious Disease
3. Military Medical Academy, Belgrade, Serbia, Institute for Transfusiology and Haemobiology
4. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Clinical Pharmacy
5. University of Kragujevac, Serbia, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research
6. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Internal medicine
7. Military Medical Academy, Belgrade, Serbia, Department of Abdominal Surgery
8. University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Radiology

Citation:
Jovanovic M, Sekulic S, Jocic M, Jurisevic M, Gajovic N, Jovanovic M, Arsenijevic N, Jovanovic M, Mijailovic M, Milosavljevic M, Jovanovic I. Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19. Int J Med Sci 2023; 20(4):530-541. doi:10.7150/ijms.80498. https://www.medsci.org/v20p0530.htm
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Abstract

Graphic abstract

Background: COVID-19 is known to disrupt immune response and induce hyperinflammation that could potentially induce fatal outcome of the disease. Until now, it is known that interplay among cytokines is rather important for clinical presentation and outcome of COVID-19. The aim of this study was to determine transcriptional activity and functional phenotype of T cells and the relationship between pro- and anti-inflammatory cytokines and clinical parameters of COVID-19 severity.

Methods: All recruited patients met criteria for COVID-19 are were divided in four groups according to disease severity. Serum levels of IL-12, IFN-γ, IL-17 and IL-23 were measured, and flow cytometry analysis of T cells from peripheral blood was performed.

Results: Significant elevation of IL-12, IFN-γ, IL-17 and IL-23 in stage IV of the disease has been revealed. Further, strong intercorrelation between IL-12, IFN-γ, IL-17 and IL-23 was also found in stage IV of the disease, marking augmented Th1 and Th17 response. Analyses of T cells subsets indicate a noticeable phenotype change. CD4+, but not CD8+ T cells expressed increased transcriptional activity through increased expression of Tbet and RORγT, accompanied with increased percentage of IFN-γ and IL-17 producing T cells.

Conclusion: Our results pose a novel hypothesis of the underlying mechanism behind deteriorating immune response in severe cases of COVID-19.

Keywords: CD4+ T cells, Th1, Th17, Tbet, RORγT, COVID-19 severity


Citation styles

APA
Jovanovic, M., Sekulic, S., Jocic, M., Jurisevic, M., Gajovic, N., Jovanovic, M., Arsenijevic, N., Jovanovic, M., Mijailovic, M., Milosavljevic, M., Jovanovic, I. (2023). Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19. International Journal of Medical Sciences, 20(4), 530-541. https://doi.org/10.7150/ijms.80498.

ACS
Jovanovic, M.; Sekulic, S.; Jocic, M.; Jurisevic, M.; Gajovic, N.; Jovanovic, M.; Arsenijevic, N.; Jovanovic, M.; Mijailovic, M.; Milosavljevic, M.; Jovanovic, I. Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19. Int. J. Med. Sci. 2023, 20 (4), 530-541. DOI: 10.7150/ijms.80498.

NLM
Jovanovic M, Sekulic S, Jocic M, Jurisevic M, Gajovic N, Jovanovic M, Arsenijevic N, Jovanovic M, Mijailovic M, Milosavljevic M, Jovanovic I. Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19. Int J Med Sci 2023; 20(4):530-541. doi:10.7150/ijms.80498. https://www.medsci.org/v20p0530.htm

CSE
Jovanovic M, Sekulic S, Jocic M, Jurisevic M, Gajovic N, Jovanovic M, Arsenijevic N, Jovanovic M, Mijailovic M, Milosavljevic M, Jovanovic I. 2023. Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19. Int J Med Sci. 20(4):530-541.

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