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Int J Med Sci 2023; 20(2):254-261. doi:10.7150/ijms.79409 This issue Cite

Research Paper

m6A modifications of circular RNAs in ischemia-induced retinal neovascularization

Yedi Zhou1,2,3, Bingyan Li1,2,3, Zicong Wang1,2,3, Wei Tan1,2,3, Jingling Zou1,2,3, Haixiang Zhou1,2,3, Yuting Cai1,2,3, Jie Liu1,2,3, Yan He1,2,3, Shigeo Yoshida4, Yun Li1,2,3✉

1. Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
2. Hunan Clinical Research Center of Ophthalmic Disease, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
3. National Clinical Research Center for Metabolic Diseases, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
4. Department of Ophthalmology, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan.

✉ Corresponding author: Yun Li, MD, PhD, Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China. Telephone: +86-731-85292175; E-mail: yun.liedu.cn.

Citation:
Zhou Y, Li B, Wang Z, Tan W, Zou J, Zhou H, Cai Y, Liu J, He Y, Yoshida S, Li Y. m6A modifications of circular RNAs in ischemia-induced retinal neovascularization. Int J Med Sci 2023; 20(2):254-261. doi:10.7150/ijms.79409. https://www.medsci.org/v20p0254.htm
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Abstract

Graphic abstract

Ischemia-induced pathological neovascularization in the retina is a leading cause of blindness in various age groups. The purpose of the current study was to identify the involvement of circular RNAs (circRNAs) methylated by N6-methyladenosine (m6A), and predict their potential roles in oxygen-induced retinopathy (OIR) in mice. Methylation assessment via microarray analysis indicated that 88 circRNAs were differentially modified by m6A methylation, including 56 hyper-methylated circRNAs and 32 hypo-methylated circRNAs. Gene ontology enrichment analysis predicted that the enriched host genes of the hyper-methylated circRNAs were involved in cellular process, cellular anatomical entity, and protein binding. Host genes of the hypo-methylated circRNAs were enriched in the regulation of cellular biosynthetic process, the nucleus, and binding. According to the Kyoto Encyclopedia of Genes and Genomes analysis, those host genes were involved in the pathways of selenocompound metabolism, salivary secretion, and lysine degradation. MeRIP-qPCR verified significant alterations in m6A methylation levels of mmu_circRNA_33363, mmu_circRNA_002816, and mmu_circRNA_009692. In conclusion, the study revealed the m6A modification alterations in OIR retinas, and the findings above shed light on the potential roles of m6A methylation in circRNA regulatory functions in the pathogenesis of ischemia-induced pathological retinal neovascularization.

Keywords: circRNA, m6A, oxygen-induced retinopathy, retinal neovascularization, RNA methylation

Citation styles

APA
Zhou, Y., Li, B., Wang, Z., Tan, W., Zou, J., Zhou, H., Cai, Y., Liu, J., He, Y., Yoshida, S., Li, Y. (2023). m6A modifications of circular RNAs in ischemia-induced retinal neovascularization. International Journal of Medical Sciences, 20(2), 254-261. https://doi.org/10.7150/ijms.79409.

ACS
Zhou, Y.; Li, B.; Wang, Z.; Tan, W.; Zou, J.; Zhou, H.; Cai, Y.; Liu, J.; He, Y.; Yoshida, S.; Li, Y. m6A modifications of circular RNAs in ischemia-induced retinal neovascularization. Int. J. Med. Sci. 2023, 20 (2), 254-261. DOI: 10.7150/ijms.79409.

NLM
Zhou Y, Li B, Wang Z, Tan W, Zou J, Zhou H, Cai Y, Liu J, He Y, Yoshida S, Li Y. m6A modifications of circular RNAs in ischemia-induced retinal neovascularization. Int J Med Sci 2023; 20(2):254-261. doi:10.7150/ijms.79409. https://www.medsci.org/v20p0254.htm

CSE
Zhou Y, Li B, Wang Z, Tan W, Zou J, Zhou H, Cai Y, Liu J, He Y, Yoshida S, Li Y. 2023. m6A modifications of circular RNAs in ischemia-induced retinal neovascularization. Int J Med Sci. 20(2):254-261.

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