Int J Med Sci 2022; 19(9):1451-1459. doi:10.7150/ijms.72988 This issue

Research Paper

The role of PTEN in puromycin aminonucleoside-induced podocyte injury

Qi Ren1,3, Shengyou Yu2, Huasong Zeng3, Huimin Xia1,3✉

1. The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, P.R. China.
2. Department of Pediatrics, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong, P.R.China.
3. Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, P.R. China.

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Citation:
Ren Q, Yu S, Zeng H, Xia H. The role of PTEN in puromycin aminonucleoside-induced podocyte injury. Int J Med Sci 2022; 19(9):1451-1459. doi:10.7150/ijms.72988. Available from https://www.medsci.org/v19p1451.htm

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Abstract

Graphic abstract

Podocytes are specialized cells of the glomerulus that play important structural and functional roles in maintaining the filtration barrier. Loss and injury of podocytes are leading factors of glomerular disease and kidney failure. Recent studies found that phosphatase and tensin homolog (PTEN) may play a critical role in maintaining the normal structure and function in podocytes. However, we still understand very little about how PTEN is regulated under podocyte injury conditions. In this study, We therefore investigated whether PTEN could play a role in podocyte injury induced by puromycin aminonucleoside (PAN), and whether dexamethasone (DEX) alleviates podocyte injury by PTEN/PI3K/Akt signaling. Our results showed that PI3K/Akt pathway was activated in podocytes exposed to PAN conditions, accompanied by down-regulation of the PTEN and microtubule-associated light chain 3 (LC3) expression.podocyte-specific knockout of PTEN significantly promoted podocyte injury, The potential renoprotection of overexpressed PTEN in podocytes was partly attributed with an improvement in autophagy and the inhibition of apoptosis.These novel findings also suggest that targeting PTEN might be a novel and promising therapeutic strategy against podocyte injury.

Keywords: Podocyte, PTEN, Proteinuria, Autophagy, Apoptosis