1. Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, P.R. China.
2. Department of Obstetrics and Gynecology, Liaoning Provincial Hospital for women and children, Shayang Street, Heping District, Shenyang 110122, P.R. China.
3. Clinical Laboratory, Shengjing Hospital of China Medical University, Shenyang, P.R. China.
4. Department of Clinical Epidemiology and Center of Evidence Based Medicine, The First Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang 110001, P.R. China.
Background: LncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) was competitive endogenous RNA (ceRNA) involved in various molecular processes for metastasis development in lung cancer. Single nucleotide polymorphisms (SNPs) in MALAT1 gene might be predictive markers for lung cancer. In our study, we selected rs619586 and rs3200401 in MALAT1 gene to explore their effects on lung cancer susceptibility.
Methods: The case-control study included 444 lung cancer cases and 460 healthy controls. Genotyping was performed by Taqman allelic discrimination method. Logistic regression, Student t-test, and Chi-square test (χ2) were used to analyze the data.
Results: The findings of the study showed that rs3200401 was significantly associated with the risk of non-small cell lung cancer (NSCLC) and lung squamous cell carcinoma (LUSC). Compared with homozygous CC genotype, CT heterozygous genotype decreased risk of NSCLC (Pa = 0.034) and LUSC (Pa = 0.025). In addition, no statistical association was detected between rs619586 and lung cancer susceptibility. The interactions between genes and cigarette smoking were discovered via crossover analysis. However, there were no remarkable gene-environment interactions in additive and multiplicative model.
Conclusion: Rs3200401 in lncRNA MALAT1 was associated with the susceptibility of non-small-cell lung cancer and lung squamous cell carcinoma. The gene-environmental (cigarette smoking) interactions were not notable.
Keywords: Lung cancer, LncRNA, MALAT1, Single nucleotide polymorphism, Interaction.