Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray

Gan, Jianfeng; Chen, Zhixian; Feng, Xuan; Wei, Zhi; Zhang, Sai; Du, Yan; Xu, Congjian; Zhao, Hongbo

doi:10.7150/ijms.65002



Theranostics

International Journal of Biological Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Med Sci 2022; 19(1):1-12. doi:10.7150/ijms.65002 This issue Cite

Research Paper

Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray

Jianfeng Gan^1,2, Zhixian Chen^1,2, Xuan Feng^1,2, Zhi Wei^1,2, Sai Zhang^1,2, Yan Du^1,2, Congjian Xu^1,2✉, Hongbo Zhao^1,2✉

1. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, People's Republic of China.
2. Department of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai 200032, People's Republic of China.

Citation:

Gan J, Chen Z, Feng X, Wei Z, Zhang S, Du Y, Xu C, Zhao H. Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray. Int J Med Sci 2022; 19(1):1-12. doi:10.7150/ijms.65002. https://www.medsci.org/v19p0001.htm

Other styles

Abstract

As a rare type of gestational trophoblastic disease, placental site trophoblastic tumor (PSTT) is originated from intermediate trophoblast cells. Long noncoding RNAs (lncRNAs) regulate numerous biological process. However, the role of lncRNAs in PSTT remains poorly understood. In the present study, expression levels of lncRNAs and mRNAs in four human PSTT tissues and four normal placental villi were investigated. The results of microarray were validated by the reverse transcription and quantitative real-time polymerase reaction (RT-qPCR) and immunohistochemistry analyses. Furthermore, GO and KEGG pathway analyses were performed to identify the underlying biological processes and signaling pathways of aberrantly expressed lncRNAs and mRNAs. We also conducted the coding-non-coding gene co-expression (CNC) network to explore the interaction of altered lncRNAs and mRNAs. In total, we identified 1247 up-regulated lncRNAs and 1013 down-regulated lncRNAs as well as 828 up-regulated mRNAs and 1393 down-regulated mRNAs in PSTT tissues compared to normal villi (fold change ≥ 2.0, p < 0.05). GO analysis showed that mitochondrion was the most significantly down-regulated GO term, and immune response was the most significantly up-regulated term. A CNC network profile based on six confirmed lncRNAs (NONHSAT114519, NR_103711, NONHSAT003875, NONHSAT136587, NONHSAT134431, NONHSAT102500) as well as 354 mRNAs was composed of 497 edges. GO and KEGG analyses indicated that interacted mRNAs were enriched in the signal-recognition particle (SRP)-dependent cotranslational protein targeting to membrane and Ribosome pathway. It contributes to expand the understanding of the aberrant lncRNAs and mRNAs profiles of PSTT, which may be helpful for the exploration of new diagnosis and treatment of PSTT.

Keywords: placental site trophoblastic tumor (PSTT), microarray analysis, lncRNA

Citation styles

APA

Gan, J., Chen, Z., Feng, X., Wei, Z., Zhang, S., Du, Y., Xu, C., Zhao, H. (2022). Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray. International Journal of Medical Sciences, 19(1), 1-12. https://doi.org/10.7150/ijms.65002.

ACS

Gan, J.; Chen, Z.; Feng, X.; Wei, Z.; Zhang, S.; Du, Y.; Xu, C.; Zhao, H. Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray. Int. J. Med. Sci. 2022, 19 (1), 1-12. DOI: 10.7150/ijms.65002.

NLM

CSE

Gan J, Chen Z, Feng X, Wei Z, Zhang S, Du Y, Xu C, Zhao H. 2022. Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray. Int J Med Sci. 19(1):1-12.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.