Int J Med Sci 2021; 18(3):768-777. doi:10.7150/ijms.48014 This issue Cite

Research Paper

circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty

Shenghui Ni1,2, Tianlong Jiang1, Shimin Hao1, Peng Luo1, Penghao Wang1, Yaser Almatari1, Yu Wang1, Zhiyu Zhang2, Lei Guo1,✉

1. Department of Orthopedic Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, 110001, P.R. China
2. Department of Orthopedic Surgery, Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, 110001, P.R. China

Citation:
Ni S, Jiang T, Hao S, Luo P, Wang P, Almatari Y, Wang Y, Zhang Z, Guo L. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int J Med Sci 2021; 18(3):768-777. doi:10.7150/ijms.48014. https://www.medsci.org/v18p0768.htm
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Abstract

Increasing evidence has demonstrated that circular RNA (circRNA) exerts important function in the pathogenesis of some diseases. While, the contributions of circRNAs to aseptic loosening after total hip arthroplasty (THA) remain largely unknown. Our research is to explore the differentially expressed circRNAs (DEcircRNAs) and elucidate complex regulated mechanism of circRNAs in aseptic loosening. The DEcircRNAs were identified by RNA sequencing (RNA-seq) analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was adopted to corroborate these DEcircRNAs. The potential function of circRNAs in aseptic loosening tissue was identified by competing endogenous RNA (ceRNA) analysis. Enrichment analysis was performed for target mRNAs and host genes of the DEcircRNAs by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 257 DEcircRNAs were obtained from RNA-seq results. Following the RT-qPCR corroboration, 6 circRNAs (hsa_circ_0007482, hsa_circ_0005232, hsa_circ_0000994, hsa_circ_0000690, hsa_circ_0058092 and hsa_circ_0004496) were selected for further analysis. By circRNA-miRNA and miRNA-mRNA prediction, 6 circRNAs, 138 miRNAs and 1667 mRNAs were identified. Then, circRNA-miRNA-mRNA network was established. The result of GO and KEGG enrichment analysis suggested that the circRNAs were related with some biological functions and pathways of aseptic loosening. A novel pathogenesis and treatment strategy about aseptic loosening after THA was revealed from our study of circRNA-miRNA-mRNA network.

Keywords: high-throughput sequencing, competitive endogenous RNA, circular RNA, total hip arthroplasty, osteolysis


Citation styles

APA
Ni, S., Jiang, T., Hao, S., Luo, P., Wang, P., Almatari, Y., Wang, Y., Zhang, Z., Guo, L. (2021). circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. International Journal of Medical Sciences, 18(3), 768-777. https://doi.org/10.7150/ijms.48014.

ACS
Ni, S.; Jiang, T.; Hao, S.; Luo, P.; Wang, P.; Almatari, Y.; Wang, Y.; Zhang, Z.; Guo, L. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int. J. Med. Sci. 2021, 18 (3), 768-777. DOI: 10.7150/ijms.48014.

NLM
Ni S, Jiang T, Hao S, Luo P, Wang P, Almatari Y, Wang Y, Zhang Z, Guo L. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int J Med Sci 2021; 18(3):768-777. doi:10.7150/ijms.48014. https://www.medsci.org/v18p0768.htm

CSE
Ni S, Jiang T, Hao S, Luo P, Wang P, Almatari Y, Wang Y, Zhang Z, Guo L. 2021. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int J Med Sci. 18(3):768-777.

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