circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty

Ni, Shenghui; Jiang, Tianlong; Hao, Shimin; Luo, Peng; Wang, Penghao; Almatari, Yaser; Wang, Yu; Zhang, Zhiyu; Guo, Lei

doi:10.7150/ijms.48014



Theranostics

International Journal of Biological Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Med Sci 2021; 18(3):768-777. doi:10.7150/ijms.48014 This issue Cite

Research Paper

circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty

Shenghui Ni^1,2, Tianlong Jiang¹, Shimin Hao¹, Peng Luo¹, Penghao Wang¹, Yaser Almatari¹, Yu Wang¹, Zhiyu Zhang², Lei Guo^1,✉

1. Department of Orthopedic Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, 110001, P.R. China
2. Department of Orthopedic Surgery, Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning, 110001, P.R. China

Citation:

Ni S, Jiang T, Hao S, Luo P, Wang P, Almatari Y, Wang Y, Zhang Z, Guo L. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int J Med Sci 2021; 18(3):768-777. doi:10.7150/ijms.48014. https://www.medsci.org/v18p0768.htm

Other styles

Abstract

Increasing evidence has demonstrated that circular RNA (circRNA) exerts important function in the pathogenesis of some diseases. While, the contributions of circRNAs to aseptic loosening after total hip arthroplasty (THA) remain largely unknown. Our research is to explore the differentially expressed circRNAs (DEcircRNAs) and elucidate complex regulated mechanism of circRNAs in aseptic loosening. The DEcircRNAs were identified by RNA sequencing (RNA-seq) analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was adopted to corroborate these DEcircRNAs. The potential function of circRNAs in aseptic loosening tissue was identified by competing endogenous RNA (ceRNA) analysis. Enrichment analysis was performed for target mRNAs and host genes of the DEcircRNAs by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 257 DEcircRNAs were obtained from RNA-seq results. Following the RT-qPCR corroboration, 6 circRNAs (hsa_circ_0007482, hsa_circ_0005232, hsa_circ_0000994, hsa_circ_0000690, hsa_circ_0058092 and hsa_circ_0004496) were selected for further analysis. By circRNA-miRNA and miRNA-mRNA prediction, 6 circRNAs, 138 miRNAs and 1667 mRNAs were identified. Then, circRNA-miRNA-mRNA network was established. The result of GO and KEGG enrichment analysis suggested that the circRNAs were related with some biological functions and pathways of aseptic loosening. A novel pathogenesis and treatment strategy about aseptic loosening after THA was revealed from our study of circRNA-miRNA-mRNA network.

Keywords: high-throughput sequencing, competitive endogenous RNA, circular RNA, total hip arthroplasty, osteolysis

Citation styles

APA

Ni, S., Jiang, T., Hao, S., Luo, P., Wang, P., Almatari, Y., Wang, Y., Zhang, Z., Guo, L. (2021). circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. International Journal of Medical Sciences, 18(3), 768-777. https://doi.org/10.7150/ijms.48014.

ACS

Ni, S.; Jiang, T.; Hao, S.; Luo, P.; Wang, P.; Almatari, Y.; Wang, Y.; Zhang, Z.; Guo, L. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int. J. Med. Sci. 2021, 18 (3), 768-777. DOI: 10.7150/ijms.48014.

NLM

CSE

Ni S, Jiang T, Hao S, Luo P, Wang P, Almatari Y, Wang Y, Zhang Z, Guo L. 2021. circRNA expression pattern and ceRNA network in the pathogenesis of aseptic loosening after total hip arthroplasty. Int J Med Sci. 18(3):768-777.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.