Int J Med Sci 2021; 18(2):432-440. doi:10.7150/ijms.47197 This issue
1. Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project Team, Chonnam National University, Gwangju, Republic of Korea.
2. Department of Oral Pathology, School of Dentistry, Institute of Biodegradable material, Institute of Oral Bioscience, Chonbuk National University, Jeonju, Republic of Korea.
3. Infectious disease Research Center, Korea research institute of bioscience & biotechnology, Daejeon, Republic of Korea.
4. Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seou, Republic of Korea.
5. Laboraotry Animal Resource Center, DGIST, Daegu, Republic of Korea.
Prevotella nigrescens is an oral pathogen that is frequently observed in the subgingival plaque of periodontitis patients. Interleukin-1β (IL-1β) is known to be involved in the immunopathology of periodontal diseases and has been implicated in the destruction of bone. In this study, we investigated the mechanism of IL-1β production by P. nigrescens in murine bone marrow-derived dendritic cells (BMDCs). Our results showed that a host receptor, Toll-like receptor 2 (TLR2), but not TLR4 is required for pro-IL-1β induction and nucleotide-binding oligomerization domain like receptor pyrin domain containing 3 (NLRP3) priming in BMDCs in response to P. nigrescens and activation of the NLRP3 inflammasome is necessary for processing of pro-IL-1β into mature IL-1β. In addition, an inhibitor assay revealed that production of reactive oxygen species, P2X7R activity, and release of cathepsin B are involved in IL-1β production in BMDCs in response to P. nigrescens.
Keywords: Prevotella nigrescens, IL-1β, TLR2, NLRP3 inflammasome, BMDCs