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Int J Med Sci 2018; 15(14):1619-1630. doi:10.7150/ijms.27647 This issue Cite

Research Paper

Decreases in Paraoxonase-1 Activities Promote a Pro-inflammatory Effect of Lipids Peroxidation Products in Non-smoking and Smoking Patients with Acute Pancreatitis

Grzegorz Marek¹, Milena Ściskalska^2✉, Zygmunt Grzebieniak¹, Halina Milnerowicz^2✉

1. Second Department of General and Oncological Surgery, Wroclaw Medical University, Wroclaw, Poland.
2. Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw, Poland.

✉ Corresponding authors: Milena Ściskalska, PhD, Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, 211 Borowska St., 50-556 Wrocław, Poland; fax: +48 71 784 01 72, e-mail: milena.topolapl, ORCID ID: 0000-0001-8976-6683 Or Halina Milnerowicz, Professor, PhD., ScD., Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, 211 Borowska St., 50-556 Wrocław, Poland; fax: +48 71 784 01 72, e-mail: halina.milnerowiczwroc.pl, ORCID ID: 0000-0002-0772-9852 More

Abstract

Aim: The study investigated the extent to which tobacco smoke exposure causes changes in lipids biochemistry through measurement blood concentrations of: paraoxonase-1 (PON-1) activities as lipid-bound enzyme into cell membrane, concentration of malonyldialdehyde (MDA), protein adducts of 4-hydroxynonenal (HNE-adducts), oxidized low density lipoproteins (oxLDL), total cholesterol (CH) and high-density lipoprotein cholesterol (HDL). Additionally, the activity of P isoform of glutathione S-transferase (GST-π) was measured.

Methods: Investigations were performed in the blood of patients with acute pancreatitis (AP) on the 1^st, 3^rd and 7^th day of hospitalization and in healthy volunteers. The activities of PON-1 forms, GST-π were determined spectrophotometrically. Concentrations of PON-1, MDA, HNE-adducts, oxLDL, HDL, CH were measured using commercial tests.

Results: Near 2-fold higher concentrations of MDA, HNE-adducts, oxLDL, correlating with inflammatory markers in AP patients compared to healthy subjects were demonstrated, which were accompanied by gradually increasing CH/HDL ratio during hospitalization. During hospital treatment, decreased activities of all PON-1 subtypes were observed in AP patients compared to healthy subjects, more pronounced in tobacco smokers. A decreased PON-1 phosphotriesterase activity in non-AP control group smokers compared to non-smokers was noted. In non-smoking AP patients GST-π activity normalized during hospitalization in contrast to smokers.

Conclusions: GST-π and PON-1 phosphotriesterase activities seem to be a sensitive marker of pro/antioxidative imbalance in smokers. Lipids peroxidation products generated during AP can intensify preexisting inflammation. Increasing stay in the hospital was associated with worsening of lipids peroxidation markers and the parameters of lipid profile, in both non-smoking and smoking AP patients, what can indicate that the oxidative-inflammatory process are not extinguished.

Keywords: acute pancreatitis, GST-π, malonylodialdehyde, paraoxonase-1, smoking.

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