Full Text | PDF

Int J Med Sci 2017; 14(13):1317-1326. doi:10.7150/ijms.20984 This issue Cite

Research Paper

Ulcer Prevention Effect Of 3,4,5-Tihydroxy-N0-[(2-Methyl-1H-Indol-3yl)Methylidene]Benzohydrazide In HCl/Ethanol-Induced Gastric Mucosal Damage In Rats

Faezeh Tayeby¹, Abbas Abdul Ameer Salman², Sareh Kamran³, Si Lay Khaing⁴, Nur'ain Binti Salehen³, Gokula Mohan A/L Duchiyanda Mohan^1✉

1. Institute of Biological Sciences, Faculty of Science, University of Malaya;
2. Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia;
3. Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;
4. Department of Obstetrics & Gynecology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

✉ Corresponding author: Dr.Gokula Mohan A/L Duchiyanda Mohan, Institute of Biological Sciences, Faculty of Science, University of Malaya University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail: g.mohanedu.my

Abstract

The newly synthesized, 3,4,5-Trihydroxy-N 0-[(2-methyl-1H-indol-3-yl)-methylidene] benzohydrazide (TIBH), is an indole and gallic acid derivative. The aim of this research investigation was to evaluate the acute toxicity and the ulcer prevention potential of TIBH in HCl/Ethanol-induced gastric ulcer rat model. Six groups of rats were orally received 5ml/kg of vehicle (1 % Carboxy methyl cellulose) for the normal and ulcer control groups each, Omeprazole (20mg/kg) for positive control, 50 mg/kg, 100 mg/kg and 200 mg/kg of TIBH for experimental groups, respectively. After one hour, instead of rats in the normal group which received 5ml/kg of 1% CMC, other groups received 5ml/kg of HCl/Ethanol. All rats were sacrificed after one additional hour. Gastric juice, gastric mucosa, morphologies of gastric ulcers and protein expressions of both control and treatment groups were evaluated. TIBH showed a ulcer prevention potential by increase of the mucus secretion, decrease of the gastric acidity, up-regulation of HSP70 protein, down-regulation of Bax protein, decrease of the lipid peroxidation and the increase of the Superoxide dismutase (SOD) activity in gastric tissue homogenate. Acute toxicity assay exposed valuable information on the safety of this compound. TIBH had a dose dependent ulcer prevention potential against HCl/Ethanol-triggered gastric ulcer.

Keywords: Gastric ulcer, Gastro-protective activity, Acute toxicity, Immunostaining.

Citation styles

©2024 Ivyspring International Publisher. Terms of use