Int J Med Sci 2017; 14(9):896-901. doi:10.7150/ijms.19933 This issue

Research Paper

Amelioration of estrogen-deficiency-induced obesity by Ocimum gratissimum

Pei-Yu Chao1*, Tsay-I Chiang2,3*, I-Chang Chang3,4, Fang-Ling Tsai5, Hsueh-Hui Lee6, Kuanghui Hsieh6, Yung-Wei Chiu7,8, Te-Jen Lai7,9, Jer-Yuh Liu5,10✉, Li-Sung Hsu11,12✉, Yang-Chia Shih13✉

1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
2. Department of Nursing, College of Medicine & Nursing, HungKuang University, Taichung, Taiwan
3. School of Medicine, Chung Shan Medical University, Taichung, Taiwan
4. Department of Orthopedic Surgery, Chung Shan Medical University, Taichung, Taiwan
5. Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan
6. Department of Laboratory Medicine, Kuang Tien General Hospital, Taichung, Taiwan
7. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
8. Emergency department and center of Hyperbaric Oxygen Therapy, Tungs' Taichung MetroHarbor Hospital, Taichung Taiwan
9. Department of Psychiatry, Chung Shan Medical Hospital, Taichung, Taiwan
10. Graduate Institute of Biomedical Sciences, China Medical University, Taichung Taiwan
11. Institute of Biochemistry, Microbiology and Immunology, Medical College, Chung-Shan Medical University, Taichung, Taiwan
12. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
13. Department of Biotechnology, Asia University, Taichung, Taiwan
* These authors contributed equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( See for full terms and conditions.
Chao PY, Chiang TI, Chang IC, Tsai FL, Lee HH, Hsieh K, Chiu YW, Lai TJ, Liu JY, Hsu LS, Shih YC. Amelioration of estrogen-deficiency-induced obesity by Ocimum gratissimum. Int J Med Sci 2017; 14(9):896-901. doi:10.7150/ijms.19933. Available from

File import instruction


Objectives: Menopausal transition in women initiates with declining estrogen levels and is followed by significant changes in their physiological characteristics. These changes often lead to medical conditions, such as obesity, which is correlated with chronic low-grade/subclinical inflammation. Ocimum gratissimum L. is a food spice or traditional herb in many countries; the plant is rich in antioxidants, which possess anti-inflammation activities and multitude of other therapeutic functions.

Methods: In this study, we evaluated effects of O. gratissimum extract (OGE) in preventing obesity by using ovariectomized (OVX) animal models to mimic menopausal women.

Methods: OVX rats showed increase in body weight and in adipocyte size in perigonadal adipose tissue (p <0.05) and decrease in uterus weight. By contrast, OGE (0.2 mg/ml) significantly reduced body weight gain and adipocyte in OVX rats and showed insignificant changes in uterus weight. Further investigation indicated that OGE exerted no influence on levels of dorsal fat, serum total cholesterol, and serum triacylglycerol and on serum biochemical factors, calcium, phosphorus, and glucose.

Conclusion: These findings suggested that OGE dietary supplements may be useful in controlling body weight of menopausal women.

Keywords: Ocimum gratissimum, obesity, ovariectomized animal models