Int J Med Sci 2013; 10(9):1157-1165. doi:10.7150/ijms.5638 This issue Cite

Research Paper

Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor

Hanna Dams-Kozlowska1,2✉, Eliza Kwiatkowska-Borowczyk1,2, Katarzyna Gryska2, Andrzej Mackiewicz1,2

1. Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, Poznan, 61-866, Poland;
2. Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan, 61-866, Poland.

Citation:
Dams-Kozlowska H, Kwiatkowska-Borowczyk E, Gryska K, Mackiewicz A. Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor. Int J Med Sci 2013; 10(9):1157-1165. doi:10.7150/ijms.5638. https://www.medsci.org/v10p1157.htm
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Abstract

Interleukin-11 (IL-11) displays megakaryopoietic activity. We constructed super-cytokine Hyper- IL11 (H11) by linking soluble IL-11 receptor α (sIL-11Rα) with IL-11, which directly targets β-receptor (gp130) signal transducing subunit. The effects of H11 on hematopoiesis with a focus on megakaryopoiesis were studied. The expansion, differentiation and type of colony formation of cord blood progenitor Lin-CD34+ cells were analyzed.

H11 was more effective than recombinant human IL-11 (rhIL-11) in enhancement of the Lin-CD34+ cells expansion and differentiation into megakaryocytes (Mk). It induced higher expression of CD41a and CD61 antigens, resulting in a substantially larger population of CD34-CD41ahighCD61high cells. H11 treatment led to increased number of small and mainly medium megakaryocyte colony formation (Mk-CFU). Moreover, it induced the formation of a small number of large colonies, which were not observed following rhIL-11 treatment. Significantly higher number of H11 derived Mk colonies released platelets-like particles (PLP). Furthermore, H11 was considerably more potent than rhIL-11 in promoting differentiation of Lin-CD43+ cells toward erythrocytes.

Our results indicate that H11 is more effective than rhIL-11 in enhancing expansion of early progenitors and directing them to megakaryocyte and erythroid cells and in inducing maturation of Mk. Thus, H11 may prove beneficial for thrombocytopenia treatment and/or an ex vivo expansion of megakaryocytes.

Keywords: interleukin 11, hyper cytokine, megakaryopoiesis, stem cells, platelets, thrombocytopenia.


Citation styles

APA
Dams-Kozlowska, H., Kwiatkowska-Borowczyk, E., Gryska, K., Mackiewicz, A. (2013). Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor. International Journal of Medical Sciences, 10(9), 1157-1165. https://doi.org/10.7150/ijms.5638.

ACS
Dams-Kozlowska, H.; Kwiatkowska-Borowczyk, E.; Gryska, K.; Mackiewicz, A. Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor. Int. J. Med. Sci. 2013, 10 (9), 1157-1165. DOI: 10.7150/ijms.5638.

NLM
Dams-Kozlowska H, Kwiatkowska-Borowczyk E, Gryska K, Mackiewicz A. Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor. Int J Med Sci 2013; 10(9):1157-1165. doi:10.7150/ijms.5638. https://www.medsci.org/v10p1157.htm

CSE
Dams-Kozlowska H, Kwiatkowska-Borowczyk E, Gryska K, Mackiewicz A. 2013. Designer Cytokine Hyper Interleukin 11 (H11) is a Megakaryopoietic Factor. Int J Med Sci. 10(9):1157-1165.

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