1. Department of Stomatology, Research Center for Oral Diseases and Biomedicine in Chongqing, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China; 2. Key Laboratory of Clinical Laboratory Science of the Ministry of Education, Chongqing Medical University, China.
✉ Corresponding author: deng63net, graceyxcom.
Citation:
Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L, Deng F. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027. https://www.medsci.org/v09p0862.htm
Dental follicle stem cells are a group of cells possessing osteogenic, adipogenetic and neurogenic differentiations, but the specific mechanism underlying the multilineage differentiation remains still unclear. Great attention has been paid to bone morphogenetic protein-9 (BMP-9) due to its potent osteogenic activity. In the present study, rat dental follicle stem cells were isolated and purified, and cells of passage 3 underwent adenovirus mediated BMP-9 gene transfection to prepare dental follicle stem cells with stable BMP-9 expression. Detection of alkaline phosphatase (ALP) and calcium deposition showed dental follicle stem cells transfected with BMP-9 gene could significantly promote the osteogenesis. In addition, SB203580 and PD98059 were employed to block the p38 mitogen-activated protein kinase (p38MAPK) and extracellular signal-regulated kinase (ERK1/2), respectively. Detection of ALP and calcium deposition revealed the BMP-9 induced osteogenic differentiation of dental follicle stem cells depended on MAPK signaling pathway.
Keywords: Dental follicle stem cells, rat recombinant adenovirus transfection, bone morphogenetic protein-9, osteogenic differentiation, signaling pathway.
Citation styles
APA
Li, C., Yang, X., He, Y., Ye, G., Li, X., Zhang, X., Zhou, L., Deng, F. (2012). Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. International Journal of Medical Sciences, 9(10), 862-871. https://doi.org/10.7150/ijms.5027.
ACS
Li, C.; Yang, X.; He, Y.; Ye, G.; Li, X.; Zhang, X.; Zhou, L.; Deng, F. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int. J. Med. Sci. 2012, 9 (10), 862-871. DOI: 10.7150/ijms.5027.
NLM
Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L, Deng F. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027. https://www.medsci.org/v09p0862.htm
CSE
Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L, Deng F. 2012. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int J Med Sci. 9(10):862-871.