Int J Med Sci 2010; 7(1):43-47. doi:10.7150/ijms.7.43 This issue

Research Paper

Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Takashi Kuwahara1, Shinya Kaneda1, Kazuyuki Shimono1, Yoshifumi Inoue2

1. Preclinical Assessment Department, Otsuka Pharmaceutical Factory, Inc., 115 Tateiwa, Naruto, Tokushima 772-8601, Japan.
2. Surgery, Kawasaki Hospital, 3-3-1 Higashiyamamachi, Hyougo-ku, Kobe 652-0042, Japan

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Kuwahara T, Kaneda S, Shimono K, Inoue Y. Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid. Int J Med Sci 2010; 7(1):43-47. doi:10.7150/ijms.7.43. Available from

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Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions without lipid. Methods: Experiment 1: A commercial TPN solution without lipid containing multivitamins (pH5.6) was used. A specific number of each test microorganism was added to each 10 mL of the TPN solution and incubated at room temperature. An aliquot of test solution was sampled and inoculated to SCD agar plates at 0, 24, and 48 hrs after the addition of the microorganisms. The number of microorganisms was counted as colony forming units. Experiment 2: The other 2 commercial TPN solutions without lipid (pH5.5) were supplemented with multivitamins. The pH values of the solutions were adjusted to about 6.0, 6.5, or 7.0 using 0.5 mol/L NaOH. The addition of microorganisms, incubation, and counting were performed in the same manner. Results: Experiment 1: S. aureus, S. marcescens, and B. cereus did not increase in the TPN solution without lipid containing multivitamins (pH5.6), but C. albicans increased rapidly. Experiment 2: The 3 bacterial species did not increase even at pH6.0, but increased at pH6.5 and increased rapidly at pH7.0 in both TPN solutions. C. albicans increased similarly at any pH. Conclusion: These results suggest that bacterial species cannot grow in TPN solutions without lipid due to the acidity (pH5.6 or lower), but Candida species can grow regardless of the acidity.

Keywords: CRBSI, microbial growth, growth of microorganism, pH, TPN solution