LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis

Li, Huan; Zhang, Yuting; Che, Mingwen; Wang, Hanmin; Li, Sutong; He, Peng; Sun, Shiren; Xu, Guoshuang; Huang, Chen; Liu, Xiaowei; Bai, Ming; Zhou, Meilan; Su, Binxiao; Zhang, Peng; He, Lijie

doi:10.7150/ijms.93547

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Int J Med Sci 2024; 21(6):1049-1063. doi:10.7150/ijms.93547 This issue Cite

Research Paper

LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis

Huan Li^1,2*, Yuting Zhang^1*, Mingwen Che^1,3*, Hanmin Wang¹, Sutong Li⁴, Peng He¹, Shiren Sun¹, Guoshuang Xu¹, Chen Huang¹, Xiaowei Liu¹, Ming Bai¹, Meilan Zhou¹, Binxiao Su^5✉, Peng Zhang^1✉, Lijie He^1✉*

1. Department of Nephrology, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
2. Department of Nephrology, the Second People's Hospital of Shaan xi Province, Xi'an, Shaanxi, China.
3. Department of Medicine, the 951 Hospital of the PLA, Korla, Xinjiang, China.
4. Department of Nephrology, Xi'an Central Hospital, Xi'an, Shaanxi, China.
5. Department of Anesthesiology and Perioperative Medicine, Department of Intensive Care Unit, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
* Authors contributed equally to this work.

Citation:

Li H, Zhang Y, Che M, Wang H, Li S, He P, Sun S, Xu G, Huang C, Liu X, Bai M, Zhou M, Su B, Zhang P, He L. LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis. Int J Med Sci 2024; 21(6):1049-1063. doi:10.7150/ijms.93547. https://www.medsci.org/v21p1049.htm

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Abstract

Peritoneal dialysis (PD), hemodialysis and kidney transplantation are the three therapies to treat uremia. However, PD is discontinued for peritoneal membrane fibrosis (PMF) and loss of peritoneal transport function (PTF) due to damage from high concentrations of glucose in PD fluids (PDFs). The mechanism behind PMF is unclear, and there are no available biomarkers for the evaluation of PMF and PTF. Using microarray screening, we found that a new long noncoding RNA (lncRNA), RPL29P2, was upregulated in the PM (peritoneal membrane) of long-term PD patients, and its expression level was correlated with PMF severity and the PTF loss. In vitro and rat model assays suggested that lncRNA RPL29P2 targets miR-1184 and induces the expression of collagen type I alpha 1 chain (COL1A1). Silencing RPL29P2 in the PD rat model might suppress the HG-induced phenotypic transition of Human peritoneal mesothelial cells (HPMCs), alleviate HG-induced fibrosis and prevent the loss of PTF. Overall, our findings revealed that lncRNA RPL29P2, which targets miR-1184 and collagen, may represent a useful marker and therapeutic target of PMF in PD patients.

Keywords: LncRNA RPL29P2, miR-1184, collagen, peritoneal dialysis, fibrosis, peritoneal transport function

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APA

Li, H., Zhang, Y., Che, M., Wang, H., Li, S., He, P., Sun, S., Xu, G., Huang, C., Liu, X., Bai, M., Zhou, M., Su, B., Zhang, P., He, L. (2024). LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis. International Journal of Medical Sciences, 21(6), 1049-1063. https://doi.org/10.7150/ijms.93547.

ACS

Li, H.; Zhang, Y.; Che, M.; Wang, H.; Li, S.; He, P.; Sun, S.; Xu, G.; Huang, C.; Liu, X.; Bai, M.; Zhou, M.; Su, B.; Zhang, P.; He, L. LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis. Int. J. Med. Sci. 2024, 21 (6), 1049-1063. DOI: 10.7150/ijms.93547.

NLM

CSE

Li H, Zhang Y, Che M, Wang H, Li S, He P, Sun S, Xu G, Huang C, Liu X, Bai M, Zhou M, Su B, Zhang P, He L. 2024. LncRNA RPL29P2 promotes peritoneal fibrosis and impairs peritoneal transport function via miR-1184 in peritoneal dialysis. Int J Med Sci. 21(6):1049-1063.

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