N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment

Zou, Luetao; Wang, Yiyang; Hu, Yanzhu; Liu, Liehua; Luo, Lei; Chen, Zhao; Zhuo, Yunyun; Li, Pei; Zhou, Qiang

doi:10.7150/ijms.90591

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Int J Med Sci 2024; 21(2):341-356. doi:10.7150/ijms.90591 This issue Cite

Research Paper

N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment

Luetao Zou, Yiyang Wang, Yanzhu Hu, Liehua Liu, Lei Luo, Zhao Chen, Yunyun Zhuo, Pei Li^✉, Qiang Zhou^✉

Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

Citation:

Zou L, Wang Y, Hu Y, Liu L, Luo L, Chen Z, Zhuo Y, Li P, Zhou Q. N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment. Int J Med Sci 2024; 21(2):341-356. doi:10.7150/ijms.90591. https://www.medsci.org/v21p0341.htm

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Abstract

The in-situ osmolarity is an important physicochemical factor that regulates cell fate of nucleus pulposus cells (NPCs). Our previous studies demonstrated that reduced N-cadherin (NCDH) expression in nucleus pulposus cells is associated with cellular damage under hyper-osmolarity microenvironment. This study was aimed at exploring the impacts of NCDH on senescence and apoptosis of NPCs, as well as the potential molecular mechanism. By comparing NPCs from patients with lumbar fractures and lumbar disc herniation, we identified a correlation between decreased NCDH expression and increased endoplasmic reticulum stress (ERS), resulting in undesirable cell fate (senescence and apoptosis). After blocking Reactive oxygen species (ROS) or ERS, it was indicated that hyper-osmolarity microenvironment induced ERS was ROS-dependent. Further results demonstrated the correlation in rat NPCs. Upregulation of NCDH expression reduced ROS-dependent ERS, thus limiting undesirable cell fates in vitro. This was further confirmed through the rat tail acupuncture injection model. NCDH overexpression successfully mitigated ERS, preserved extracellular matrix production and alleviating intervertebral disc degeneration in vivo. Together, NCDH can alleviate senescence and apoptosis of NPCs by suppressing ROS-dependent ERS via the ATF4-CHOP signaling axis in the hyper-osmolarity microenvironment, thus highlighting the therapeutic potential of NCDH in combating degenerative disc diseases.

Keywords: N-cadherin, nucleus pulposus cells, intervertebral disc degeneration, endoplasmic reticulum stress, hyper-osmolarity microenvironment

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APA

Zou, L., Wang, Y., Hu, Y., Liu, L., Luo, L., Chen, Z., Zhuo, Y., Li, P., Zhou, Q. (2024). N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment. International Journal of Medical Sciences, 21(2), 341-356. https://doi.org/10.7150/ijms.90591.

ACS

Zou, L.; Wang, Y.; Hu, Y.; Liu, L.; Luo, L.; Chen, Z.; Zhuo, Y.; Li, P.; Zhou, Q. N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment. Int. J. Med. Sci. 2024, 21 (2), 341-356. DOI: 10.7150/ijms.90591.

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CSE

Zou L, Wang Y, Hu Y, Liu L, Luo L, Chen Z, Zhuo Y, Li P, Zhou Q. 2024. N-cadherin Alleviates Apoptosis and Senescence of Nucleus Pulposus Cells via Suppressing ROS-dependent ERS in the Hyper-osmolarity Microenvironment. Int J Med Sci. 21(2):341-356.

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