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Int J Med Sci 2024; 21(1):137-150. doi:10.7150/ijms.88298 This issue Cite

Research Paper

Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis

Shanyu Li1#, Hongxiao Wu2#, Feng Wang1, Lingchi Kong1, Yifan Yu1, Rongtai Zuo1, Haoyu Zhao1, Jia Xu1✉, Qinglin Kang1✉

1. Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
2. Department of Orthopedics, Dongying People's Hospital, Dongying, Shandong, PR China.
# These authors contributed equally to this work.

✉ Corresponding authors: Qinglin Kang, Jia Xu, Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, PR China; Tel: +86-21-64369181; Fax: +86-21-64701361; E-mail: orthokan More

Citation:
Li S, Wu H, Wang F, Kong L, Yu Y, Zuo R, Zhao H, Xu J, Kang Q. Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. Int J Med Sci 2024; 21(1):137-150. doi:10.7150/ijms.88298. https://www.medsci.org/v21p0137.htm
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Abstract

Graphic abstract

Background: Focal adhesion kinase (FAK) is activated by mechanical stimulation and plays a vital role in distraction osteogenesis (DO), a well-established but lengthy procedure for repairing large bone defects. Both angiogenesis and osteogenesis contribute to bone regeneration during DO. However, the effects of ZINC40099027 (ZN27), a potent FAK activator, on angiogenesis, osteogenesis, and bone regeneration in DO remain unknown.

Methods: The angiogenic potential of human umbilical vein endothelial cells (HUVECs) was evaluated using transwell migration and tube formation assays. The osteogenic activity of bone marrow mesenchymal stem cells (BMSCs) was assessed using alkaline phosphatase (ALP) and alizarin red s (ARS) staining. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunofluorescence staining were used to assay angiogenic markers, osteogenic markers, and FAK-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling. In vivo, a rat tibia DO model was established to verify the effects of ZN27 on neovascularization and bone regeneration using radiological and histological analyses.

Results: ZN27 promoted the migration and angiogenesis of HUVECs. Additionally, ZN27 facilitated the osteogenic differentiation of BMSCs, as revealed by increased ALP activity, calcium deposition, and expression of osteogenesis-specific markers. The ERK1/2-specific inhibitor PD98059 significantly hindered the effects of ZN27, suggesting the participation of FAK-ERK1/2 signaling in ZN27-enhanced angiogenesis and osteogenesis. As indicated by improved radiological and histological features, ZN27 induced active angiogenesis within the distraction area and accelerated bone regeneration in a rat DO model.

Conclusion: Our results show that ZN27 targets FAK-ERK1/2 signaling to stimulate both angiogenesis and osteogenesis, and ZN27 accelerates bone regeneration in DO, suggesting the therapeutic potential of ZN27 for repairing large bone defects in the mechanobiological environment during DO.

Keywords: Distraction osteogenesis, Focal adhesion kinase, ZINC40099027, Angiogenesis, Bone regeneration, Mechanical stimulation

Citation styles

APA
Li, S., Wu, H., Wang, F., Kong, L., Yu, Y., Zuo, R., Zhao, H., Xu, J., Kang, Q. (2024). Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. International Journal of Medical Sciences, 21(1), 137-150. https://doi.org/10.7150/ijms.88298.

ACS
Li, S.; Wu, H.; Wang, F.; Kong, L.; Yu, Y.; Zuo, R.; Zhao, H.; Xu, J.; Kang, Q. Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. Int. J. Med. Sci. 2024, 21 (1), 137-150. DOI: 10.7150/ijms.88298.

NLM
Li S, Wu H, Wang F, Kong L, Yu Y, Zuo R, Zhao H, Xu J, Kang Q. Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. Int J Med Sci 2024; 21(1):137-150. doi:10.7150/ijms.88298. https://www.medsci.org/v21p0137.htm

CSE
Li S, Wu H, Wang F, Kong L, Yu Y, Zuo R, Zhao H, Xu J, Kang Q. 2024. Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. Int J Med Sci. 21(1):137-150.

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