ISSN: 1449-1907International Journal of Medical Sciences
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Int J Med Sci 2023; 20(13):1744-1754. doi:10.7150/ijms.87830 This issue Cite
Research Paper
Increased Expression of Tetraspanins, ALIX and HSP-70 in the Placenta Tissue of Women with Chronic Venous Disease during Pregnancy
1. Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, Alcala de Henares, Spain.
2. Ramón y Cajal Institute of Sanitary Research (IRYCIS), Madrid, Spain.
3. University Defense Center of Madrid (CUD), Madrid, Spain.
4. Unit of Biochemistry and Molecular Biology, Centro de Investigación Biomédica en Red en El Área Temática de Enfermedades Hepáticas (CIBEREHD), Department of System Biology, University of Alcalá, Alcala de Henares, Spain.
5. Department of General and Digestive Surgery, University Hospital Príncipe de Asturias, 28805 Madrid, Spain.
6. Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá, 28801 Alcala de Henares, Spain.
7. Department of Obstetrics and Gynecology, University Hospital Gregorio Marañón, Madrid, Spain.
8. Health Research Institute Gregorio Marañón, Madrid, Spain.
9. Department of Public and Maternal and Child Health, School of Medicine, Complutense University of Ma-drid, Madrid, Spain.
10. Immune System Diseases-Rheumatology, Oncology Service an Internal Medicine, Centro de Investigación Biomédica en Red en El Área Temática de Enfermedades Hepáticas (CIBEREHD), University Hospital Príncipe de Asturias, Alcala de Henares, Spain.
11. Pathological Anatomy Service, Central University Hospital of Defence-UAH, 28047 Madrid, Spain.
*These authors contributed equally to this work.
#These authors are senior authors.
Abstract
Chronic venous disease (CVD) is a complex and common vascular disorder characterized by increased blood pressure and morpho-functional changes in the venous system like varicose veins. Pregnancy is one of the main risk factors for suffering from this condition. Despite the consequences of CVD during pregnancy remains to be fully understood, compelling evidence support that this condition represents an important stress for the mother and the fetus, leading to significant histopathological changes in the placenta. Tetraspanins (CD9, CD63, and CD81), ALG-2-interacting protein X (Alix), and heat-shock protein (HSP-70) are cellular components involved in multiple biological processes under homeostatic and disease conditions. Despite some studies that have evidence of their relevance in the placenta tissue and pathological pregnancies, there is limited knowledge regarding their role in pregnancy-associated CVD. In this sense, the present work aims to analyze gene and protein expression of these components in the placenta of women with CVD (n=62) in comparison to healthy women (n=52) through RT-qPCR and immunohistochemistry, respectively. Our results show an increased gene and protein expression of the different studied markers, suggesting their potential involvement in the pathological environment of the placenta of women who undergo CVD during pregnancy. In this sense, further studies should be directed to deep into the potential implications of these changes to understand the effects and consequences of this condition in maternofetal wellbeing.
Keywords: Chronic venous disease (CVD), Pregnancy, placenta, Tetraspanins, ALG-2 interacting protein (ALIX), Heat shock protein 70 (HSP-70)
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