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Int J Med Sci 2023; 20(8):1079-1090. doi:10.7150/ijms.83828 This issue Cite

Research Paper

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

Yingfei Long, Yifei Ren, Qinglv Wei, Youchaou Mobet, Yujiao Liu, Hongyan Zhao, Tao Liu✉, Lei Cheng✉, Ping Yi✉

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China.

✉ Corresponding author: Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China.

Citation:
Long Y, Ren Y, Wei Q, Mobet Y, Liu Y, Zhao H, Liu T, Cheng L, Yi P. NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. Int J Med Sci 2023; 20(8):1079-1090. doi:10.7150/ijms.83828. https://www.medsci.org/v20p1079.htm
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Abstract

Graphic abstract

N4-acetylcytidine (ac4C) is a lately discovered nucleotide modification that has been shown to be closely implicated in cancer. N-acetyltransferase10(NAT10) acts as an enzyme that regulates mRNA acetylation modifications. Currently, the role of NAT10-mediated RNA acetylation modification in cervical cancer remains to be elucidated. On the basis of transcriptome analysis of TCGA and GEO open datasets (GSE52904, GSE29570, GSE122697), NAT10 is upregulated in cervical cancer tissues and correlated with poor prognosis. Knockdown of NAT10 suppressed the cell proliferation, invasion, and migration of cervical cancer cells. The in vivo oncogenic function of NAT10 was also confirmed in xenograft models. Combined RNA-seq and acRIP-seq analysis revealed HNRNPUL1 as the target of NAT10 in cervical cancer. NAT10 positively regulate HNRNPUL1 expression by promoting ac4C modification and stability of HNRNPUL1 mRNA. Furthermore, depletion of HNRNPUL1 suppressed the cell division, invasion, and migration of cervical cancer. HNRNPUL1 overexpression partially restored cellular function in cervical cancer cells with NAT10 knockdown. Thus, this study demonstrates that NAT10 contributes to cervical cancer progression by enhancing HNRNPUL1 mRNA stability via ac4C modification, and NAT10-ac4C-HNRNPUL1 axis might be a potential target for cervical cancer therapy.

Keywords: N4-acetylcytidine (ac4C), cervical cancer, HNRNPUL1, mRNA stability, NAT10

Citation styles

APA
Long, Y., Ren, Y., Wei, Q., Mobet, Y., Liu, Y., Zhao, H., Liu, T., Cheng, L., Yi, P. (2023). NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. International Journal of Medical Sciences, 20(8), 1079-1090. https://doi.org/10.7150/ijms.83828.

ACS
Long, Y.; Ren, Y.; Wei, Q.; Mobet, Y.; Liu, Y.; Zhao, H.; Liu, T.; Cheng, L.; Yi, P. NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. Int. J. Med. Sci. 2023, 20 (8), 1079-1090. DOI: 10.7150/ijms.83828.

NLM
Long Y, Ren Y, Wei Q, Mobet Y, Liu Y, Zhao H, Liu T, Cheng L, Yi P. NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. Int J Med Sci 2023; 20(8):1079-1090. doi:10.7150/ijms.83828. https://www.medsci.org/v20p1079.htm

CSE
Long Y, Ren Y, Wei Q, Mobet Y, Liu Y, Zhao H, Liu T, Cheng L, Yi P. 2023. NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. Int J Med Sci. 20(8):1079-1090.

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