1. Division of Cardiology, Department of internal medicine, Chung-Shan Medical University Hospital Taichung 40201.
2. Institute of Medicine, School of Medicine, Chung Shan Medical University, Taichung 40201.
3. Graduate Institute of Bio-industry Management, College of Agriculture and Nature Resources, National Chung Hsing University, Taichung 40227.
4. Department of Pharmacy, Chung Shan Medical University Hospital, Taichung 40201.
5. Department of Occupational Safety and Health, College of Health Care and Management, Chung Shan Medical University, Taichung 40201.
6. Department of Physiology, School of Medicine, Chung Shan Medical University, Taichung 40201.
7. Department of Early Childhood Educare, College of Health Sciences, TransWorld University, Douliu City, Yunlin County 64063.
8. Department of Medical Research, Chung Shan Medical University Hospital, Taichung 40201, Taiwan.
*Dr. Hung-Chih Ting and Dr. Ching-Han Yu have the same contribution to this paper.
Background: Resveratrol, a natural antioxidant polyphenol, has the functions of anti-inflammation, anti-cancer, liver protection and cardioprotection. Microorganism biotransformation-produced resveratrol (MBR) product shows higher purity than the natural source of resveratrol and costs less than the chemically synthesized resveratrol. The aim of the present study was to investigate the protective effects of MBR in hamsters treated with a high-fat diet (HFD).
Methods: MBR was obtained by the fermentative process of piceid. Hamsters were randomly divided into four groups: HFD plus oral administration of MBR 0 (C), 5 (L), 20 (M) or 50 mg/kg (H), respectively. After six-week of treatment, hamsters were sacrificed, and tissues were collected for further analysis.
Results: MBR at these three dosages did not influence the appetite or growth of the hamsters. Liver enzymes, blood glucose, total cholesterol, triglyceride, and liver weight were significantly reduced in the MBR groups than in the control group. Additionally, high-density lipoprotein-cholesterol (HDL-C) was also elevated in all MBR groups. On the other hand, serum low-density lipoprotein-cholesterol (LDL-C) was decreased in the MBR groups. Triglyceride (TG) in liver tissue and fatty liver level were lower in group H. Memory-associated proteins, phosphorylation of calmodulin-dependent protein kinase II (p-CaMK II) and synaptophysin (SYP), were increased in the brains of MBR groups.
Conclusion: The high yield- and short procedure-produced MBR has the potential to protect animals fed with HFD from hyperlipidemia, hepatic steatosis, hyperglycemia, and synaptic impairment, which might be beneficial for patients with these types of diseases.
Keywords: hepatic steatosis, hyperlipidemia, microorganism biotransformation-produced resveratrol, synaptic impairment.