Int J Med Sci 2022; 19(8):1235-1240. doi:10.7150/ijms.73007 This issue

Research Paper

Raddeanin A (RA) reduced acute inflammatory injury in mouse experimental cerebral hemorrhage by suppression of TLR4

Bo Hei, Jia Ouyang, Jingru Zhou, Dongliang Wang, Zeyu Miao, Ru-en Liu

Department of neurosurgery, Peking University People's Hospital, Peking University, Beijing, China

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Citation:
Hei B, Ouyang J, Zhou J, Wang D, Miao Z, Liu Re. Raddeanin A (RA) reduced acute inflammatory injury in mouse experimental cerebral hemorrhage by suppression of TLR4. Int J Med Sci 2022; 19(8):1235-1240. doi:10.7150/ijms.73007. Available from https://www.medsci.org/v19p1235.htm

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Abstract

Graphic abstract

Spontaneous intracerebral hemorrhage (ICH) is associated with high mortality and disability rates. The microglia-induced inflammatory response is a critical factor determining brain tissue damage after ICH. Raddeanin A (RA) is a natural triterpenoid compound with anti-inflammatory effects, although its effects on ICH and the underlying molecular mechanism have not been elucidated. In this study, we found that RA reduced the volume of cerebral hematoma and cerebral edema, attenuated neuronal apoptosis and improved the behavioral indices in a murine model of acute cerebral hemorrhage. Mechanistically, RA downregulated the TLR4-mediated pro-inflammatory effectors, reduced infiltration of microglia in peri-intracerebral hemorrhage and inhibited apoptosis of neurons co-cultured with activated microglia. In conclusion, RA can alleviate ICH-related tissue damage and promote the recovery of neuronal function by suppressing microglia-induced inflammation and apoptosis.

Keywords: Intracerebral hemorrhage, Raddeanin A, inflammation, microglia, TLR4