Int J Med Sci 2022; 19(4):609-617. doi:10.7150/ijms.70614 This issue Cite

Research Paper

Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro

Xiangdong Li1, Zhiyuan Wang2, Heyu Meng1, Fanbo Meng1✉, Ping Yang1✉

1. Cardiovascular Department of China-Japan Union Hospital of Jilin University.
2. Ultrasound Department of China-Japan Union Hospital of Jilin University.

Citation:
Li X, Wang Z, Meng H, Meng F, Yang P. Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro. Int J Med Sci 2022; 19(4):609-617. doi:10.7150/ijms.70614. https://www.medsci.org/v19p0609.htm
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Abstract

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Background: Previous microarray analysis on peripheral blood leukocytes from three patients with acute myocardial infarction (AMI) showed that elevated expression of membrane bound o-acyltransferase domain containing 7(MBOAT7) relative to control. To further verify these findings, we investigated more patients and explored the possible mechanisms in vitro.

Objective: To study alterations in MBOAT7 expression in leukocytes after AMI, and to explore the relationship between MBOAT7 and lipid metabolism pathways in hepatocytes in vitro.

Methods: Ninety patients with AMI and 90 controls were recruited from the Han population in Northeast China. RT-fluorescent PCR was used to measure MBOAT7 mRNA levels. MBOAT7 interference and overexpression vectors were constructed and transfected into L-02 hepatocytes and expression was examined by RT-qPCR and western blotting. The expression of SCAP, LDLR, HMGCR, ACAT1, ABCA1, SREBP1, ACC, FAS, SCD, and PPARγ in the lipid metabolism pathway were investigated by RT-qPCR. Triglyceride and cholesterol levels were measured by ELISA.

Results: It was found that MBOAT7 mRNA levels were elevated in the leukocytes of patients with AMI. Hepatocytes were successfully transfected, shown by attenuated MBOAT7 mRNA levels in the silenced group (0.41±0.04 vs 1.01±0.07 for control, P=0.0019 <0.01) and raised levels in the overexpressing cells (23.29±0.39 vs 1.00±0.06 for control, P <0.0001). These results were confirmed by western blotting. Expression of the lipid metabolism-related genes was altered in response to MBOAT7 expression. Triglyceride levels increased after MBOAT7 silencing (118.40 ± 2.26 vs 70.54 ± 0.25 for control, P<0.0001), as did those of cholesterol (628.30 ± 8.89 vs 544.70 ± 11.04, P = 0.0041) but were not altered on MBOAT7 overexpression.

Conclusion: MBOAT7 did not affect the metabolism of triglycerides in hepatocytes through fatty acid synthesis and decomposition pathways. The MBOAT7 level in the peripheral blood can be used as a marker for acute myocardial infarction but cannot be used as a single therapeutic target to regulate lipid metabolism.

Keywords: Myocardial Infarction, MBOAT7, Marker, Lipid Metabolism


Citation styles

APA
Li, X., Wang, Z., Meng, H., Meng, F., Yang, P. (2022). Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro. International Journal of Medical Sciences, 19(4), 609-617. https://doi.org/10.7150/ijms.70614.

ACS
Li, X.; Wang, Z.; Meng, H.; Meng, F.; Yang, P. Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro. Int. J. Med. Sci. 2022, 19 (4), 609-617. DOI: 10.7150/ijms.70614.

NLM
Li X, Wang Z, Meng H, Meng F, Yang P. Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro. Int J Med Sci 2022; 19(4):609-617. doi:10.7150/ijms.70614. https://www.medsci.org/v19p0609.htm

CSE
Li X, Wang Z, Meng H, Meng F, Yang P. 2022. Expression of Membrane Bound O-Acyltransferase Domain Containing 7 after Myocardial Infarction and its Role in Lipid Metabolism in vitro. Int J Med Sci. 19(4):609-617.

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