Int J Med Sci 2021; 18(15):3403-3411. doi:10.7150/ijms.60617 This issue
1. Department of Otolaryngology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, China.
2. NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, 200031, PR China.
3. Nursing Department, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, China.
# Co-first authors
Herein, we purposed to explore whether hypoxia triggers proliferation of cholesteatoma keratinocytes via the PI3K-Akt signaling cascade. Cells were inoculated with different concentration of CoCl2. The proliferation and cellular HIF-1α, p-PDK1 and p‑Akt expression levels of cholesteatoma keratinocytes were assessed in vitro. Hypoxia escalated cell proliferation via upregulating p-PDK1 and p‑Akt expressions. Specific inhibitor of the PI3K-Akt signaling cascade, LY294002 markedly inhibited the expression of p‑Akt and significantly reduces the hypoxia‑induced proliferation of cholesteatoma keratinocytes. Our data provides research evidence confirming that hypoxia participates in the onset and progress of cholesteatoma.
Keywords: hypoxia, cholesteatoma, HIF-1α, cell proliferation, PI3K/Akt signaling pathway