Int J Med Sci 2021; 18(4):902-910. doi:10.7150/ijms.50984 This issue
1. Department of Orthopedics, the Fourth Affiliated Hospital of China Medical University, No.4 East Chongshan Road, Shenyang, 110032, P.R. China
2. Center for Translational Medicine, the Fourth Affiliated Hospital of China Medical University, No.4 East Chongshan Road, Shenyang, 110032, P.R. China
# These authors contribute equally to this work.
The pathogenesis of hallux valgus is not clearly understood. However, genetics research about hallux valgus is rare. Therefore, the present study aimed to explore the pathogeny of hallux valgus from the perspective of genetics. Human samples were collected from normal bone tissue and hallux valgus region bone tissue. The bone samples were studied using real time-PCR, western blot and immunohistochemical. Lentivirus-mediated miR-182 transfected osteoblasts and tested the expression of FGF9 mRNA with real time-PCR. To test alkaline phosphatase activity, number of calcium nodules and proliferation of osteoblast with enzymatic activity analysis, calcium nodules stained and MTT assay. We found that (1) FGF9 expressed in hallux valgus region bone tissue was significantly higher than normal bone tissue. (2) miR-182 expression levels in hallux valgus region bone tissue were notably lower than those of normal bone tissue. (3) miR-182 could negatively regulate the expression of FGF9 in osteoblasts. (4) FGF9 may enhance osteoblasts proliferation. We have demonstrated that miR-182 promotes the formation of bone by targeting FGF9, implicating an essential role of miR-182 in the etiology of hallux valgus. Moreover, miR-182 might potentially be a therapeutic target for hallux valgus treatment.
Keywords: hallux valgus, FGF9, miR-182, osteoblast, alkaline phosphatase