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Int J Med Sci 2020; 17(10):1307-1314. doi:10.7150/ijms.43140 This issue Cite

Review

The Role of Histone Acetyltransferases and Histone Deacetylases in Photoreceptor Differentiation and Degeneration

Meng Zhao^1,2, Ye Tao^1,3 , Guang-Hua Peng^1,2

1. Laboratory of Visual Cell Differentiation and Regulation, Basic Medical College, Zhengzhou University, Zhengzhou 450001, China.
2. Department of Pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, China.
3. Department of Physiology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, China.

✉ Corresponding authors: ghpedu.cn; panghtoyezzedu.cn; Tel.: +86-0371-6778-1855 (G.-H.P.); Fax.: +86-0371-6778-1959 (G.-H.P.)

Abstract

Photoreceptors are critical components of the retina and play a role in the first step of the conversion of light to electrical signals. The differentiation and degeneration of photoreceptors are regulated by specific genes and proteins. With the development of epigenetic approaches, scientists have discovered that histone modifications, such as acetylation, methylation, ubiquitylation, and phosphorylation, may modulate the processes of photoreceptor differentiation and degeneration. Histone acetylation is regulated by two opposing classes of enzymes, namely, histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups to and from target histones, respectively, causing changes in transcriptional activity. Herein, we review the effects of HATs and HDACs on the differentiation and degeneration of photoreceptors and discuss the underlying mechanisms of these effects.

Keywords: HDAC, HAT, photoreceptor, differentiation, degeneration

Introduction

The retina originates from the central nervous system and is defined as the “window to the brain.” The retina contains five major types of neuronal cells (photoreceptors, horizontal cells, bipolar cells, amacrine cells, and ganglion cells), as well as Müller glia cells, astrocytes, and microglia. In mammals, these cells are distributed throughout five laminar retinal layers, namely, the outer nuclear layer (ONL), outer plexiform layer (OPL), inner nuclear layer, inner plexiform layer (IPL), and ganglion cell layer^{1, 2}. Photoreceptors, situated in the ONL and OPL of the vertebrate retina, can convert light into a neuronal signal, which is recognized as the beginning of visual processing. Photoreceptors comprise two types of neuronal cells, i.e., rods, which are responsible for dim light and night vision, and cones, which are responsible for daylight vision. Retinal rods are present in much larger numbers than cones. The nuclei of these cells are located in the ONL, and cone nuclei are distinctly larger and less condensed than rod nuclei³. Rod and cone photoreceptors establish synapses with bipolar cells in the OPL, while horizontal cells regulate synapse transmission. Cone bipolar cells interact with retinal ganglion cells and amacrine cells in the IPL. Amacrine cells are linked to ganglion cells, which are output neurons in the retina, projecting neural signals onto visual centers in the brain cortex².

The transcription and translation processes in cone and rod photoreceptor cells are regulated by multiple transcriptional factors, such as the neural retina leucine zipper (NRL) protein, cone-rod homeobox (CRX) protein, orthodenticle homolog 2 (OTX2), nuclear receptor subfamily 2 group E member 3 (NR2E3) protein, and the retinal homeobox (RAX) protein; they also involve many pathways, such as the Sonic hedgehog (Shh), Notch, and thyroid hormone pathways^4-8. Although the exact role of epigenetics in photoreceptor cell development and degeneration is not well known, epigenetic studies may be of great importance⁹. Epigenetics is the study of mitotically and/or meiotically heritable changes in gene function, which do not involve changes in the DNA sequence¹⁰. Posttranslational modifications of histone proteins, such as acetylation, methylation, ubiquitylation, and phosphorylation, can alter the structure of the chromatin around human genes. In general, according to its transcriptional state, the chromatin can be divided into actively transcribed euchromatin and transcriptionally inactive heterochromatin¹¹. Histone acetylation, which mainly occurs at lysine residues in the N-terminal tail of the core histones H2A, H2B, H3, and H4, is associated with various physiological and pathological processes through changes in the chromatin structure and gene expression levels¹². The homeostasis of the acetylation state is regulated by two classes of antagonizing histone-modifying enzymes, histone acetyltransferases (HATs) and histone deacetylases (HDACs). These histone-modifying enzymes can add or remove acetyl groups to or from target histones, respectively, leading to an increase or decrease in transcriptional activity and thus regulating the expression of target genes^{13, 14}. Emerging studies show that HATs and HDACs play critical roles in the differentiation and degeneration of photoreceptors and indicate that HDAC inhibitors may have a therapeutic potential in some retinopathies.

Therefore, this review discusses the roles of HATs and HDACs in photoreceptor differentiation and degeneration, reviews studies on the underlying mechanisms of these effects, and highlights the therapeutic potential of targeting HATs and HDACs for the treatment of ophthalmic diseases caused by photoreceptor degeneration.

HATs and HDACs

Mammalian HATs are divided into three families. The Gnat5 family, whose main substrates are histone proteins, includes Gcn5, PCAF, Hat1, Elp3, and Hpa2; the MYST family, whose main substrate is the H4 protein, comprises Esa1, MOF, Sas2, Sas3, MORF, Tip60, and Hbo1; and the p300/CBP family comprises p300 and CBP, which act on H3 and H4¹⁵. Recent research has revealed that HATs contribute to nucleosome assembly, DNA repair and transcription, and protein deposition^{12, 16} (Table 1).

In total, 18 human HDACs have been identified so far. Based on developmental genetics and sequence similarity with the yeast proteins RPD3, HDA1, SIR2, and HOS3¹⁷, human HDACs have been grouped into the following four classes: class I (HDAC1, HDAC2, HDAC3, and HDAC8); class II, which is further divided into two subclasses: IIa (HDAC4, HDAC5, HDAC7, and HDAC9) and IIb (HDAC6 and HDAC10); class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, and SIRT7); and class IV (HDAC11)^{18, 19}. Class I, II, and IV enzymes are Zn⁺-dependent protein deacetylases, while class III enzymes are NAD⁺-dependent protein deacetylases. Class I HDACs are mainly located in the nucleus and contribute to cell development and differentiation, especially in neurogenesis. Class II HDACs are present both in the cytoplasm and nucleus and collaborate with class I proteins, whereas class III HDACs are independent and located in the nucleus, mitochondria, or cytoplasm^{20, 21} (Table 2).

HDACs, HATs, and differentiation of photoreceptor cells

The differentiation of photoreceptor cells starts from retinal progenitor cells. First, multipotent retinal progenitor cells proliferate into retinal progenitor cells. Next, progenitor genes are gradually silenced, and photoreceptor-specific genes are expressed as photoreceptor precursors, which differentiate into mature photoreceptor cells. Finally, synapses are formed through axonal growth and outer segment formation²². In humans, photoreceptors are generated before birth, and cone receptor formation occurs 2 weeks before rod receptor formation²³.

Recent studies have revealed underlying regulatory mechanisms of the HAT and HDAC effects on gene expression and photoreceptor differentiation. c-Jun N-terminal kinase 1 (JNK1) can directly interact with NRL, phosphorylating its serine-50 residue and enhancing NRL activity. Phosphorylated NRL binds to the rhodopsin and Ppp2r5c promoters, activating their transcription through the recruitment of the HIV Tat-interacting protein (Tip60) to promote histone H3/H4 acetylation²⁴. These data suggest that the cooperation among NRL, JNK1, and Tip60 plays a regulatory role in photoreceptor differentiation.

 Table 2 

HDAC family members^{18, 94, 95}

Class	Subtype	Protein domain	Number of amino acids	Protein size (kDa)	Localization	Yeast homolog
Ⅰ	HDAC1		482	58	Nucleus	RPD3
	HDAC2		488	59	Nucleus
	HDAC3		428	50	Nucleus, cytoplasm
	HDAC8		377	44	Nucleus
Ⅱa	HDAC4		1,084	120	Nucleus, cytoplasm	Hda1
	HDAC5		1,122	130	Nucleus, cytoplasm
	HDAC7		912	110	Nucleus, cytoplasm
	HDAC9		1,011	160	Nucleus, cytoplasm
Ⅱb	HDAC6		1,215	160	Nucleus, cytoplasm
	HDAC10		669	70	Nucleus, cytoplasm
Ⅲ	Sirt1		747	120	Nucleus	Sir2
	Sirt2		389	45	Cytoplasm
	Sirt3		399	28	Mitochondria
	Sirt4		314	35	Mitochondria
	Sirt5		310	36	Mitochondria
	Sirt6		355	39	Nucleus
	Sirt7		400	48	Nucleus
Ⅳ	HDAC11		347	39	Nucleus	HOS3

Catalytic domain MEF-binding domain

Another study illustrated the role of HATs in photoreceptor differentiation during retinal development by assessing CRX expression. One way of activating the transcription of this protein is to recruit the transcriptional coactivators p300 and CBP to acetylate promoter-bound histones. A conditional knockdown of p300 and CBP in differentiating rods or cones reduced the histone H3/H4 acetylation and consequently decreased the transcription levels of photoreceptor-specific genes, disrupting the function and structure of these cells²⁵.

From postnatal day 2 to 10, the inhibition of class I and II HDACs with trichostatin A (TSA) was shown to cause the numbers of rod photoreceptors to drop; it also caused transcription factors that are essential for rod differentiation, such as OTX2, CRX, and NRL, as well as NeuroD1 expression (necessary for rod photoreceptor survival) to decrease within 3 h²⁶. These results indicated that HDACs could promote the differentiation of rod photoreceptors and played a key role in deciding the fate of photoreceptor progenitors, although further efforts are still needed to elucidate the exact role of each HDAC. Using a specific HDAC1 inhibitor in mice, Ferreira et al.²⁷ demonstrated that the transcription levels of Vsx2, Hes1, and other progenitor-specific genes remained stable because their promoters were acetylated. By contrast, the expression levels of Nrl, Rho, and other rod-specific genes decreased because of a reduction in histone acetylation. These authors tested three histone sites and discovered that the acetylation of H3K9 and H4K12 increased, while that of H3K27 did not change upon HDAC1 inhibition. These findings suggest that HDAC1 is a key protein in the process leading a progenitor cell to form a terminally differentiated rod photoreceptor; however, HDAC3 did not show similar functions in the differentiation of rod photoreceptors after birth²⁷.

Cell division is the basis of growth, development, and reproduction of individuals in multicellular organisms. The destiny of cells is related to the stage of the cell cycle in which they are found. Cells with differentiation potential stay in the G0 phase and reenter a new cell cycle to become differentiated when induced^{28, 29}. The differentiation of retinal progenitor cells into retinal neurons is regulated during development by cell-cycle molecules. Therefore, it is essential to investigate the cell cycle of photoreceptor cells and the mechanism of its regulation by HATs and HDACs. Using hdac1 mutant models to study the zebrafish retina, Stadler et al.³⁰ found that HDAC1 was essential for the cell-cycle exit during retina differentiation, which was accompanied by a reduction in the cyclin D and E levels. Cyclins D and E are the drivers of cell-cycle progression, and their regulation is region and species specific. Cyclin D1 interacts with the Notch1 gene, where it recruits the CBP HAT during mouse retinal development³¹. The retinoblastoma protein (Rb) can bind to the tumor suppressor protein E2F and form a cell-cycle regulator complex, which functions alongside HDACs³². These studies indicate that HDACs and HATs can affect the cell cycle of photoreceptors during their development; however, more in-depth research is still required in this field.

Degeneration of photoreceptor cells

DNA sequences, transcription patterns, and translation must function in an error-free and coherent manner to maintain the normal function and homeostasis of photoreceptors. Therefore, gene mutations, transcriptional disorders, and microenvironmental changes can lead to photoreceptor loss or dysfunction. Generally, photoreceptor diseases can be classified as nature and nurture types. The best-studied primary inherited rod degenerative diseases, which are followed by cone degeneration, are retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA)³³. Primary inherited cone degenerative diseases include Stargardt's and Best's diseases, achromatopsia, and cone dystrophies. Cone dystrophies are caused by at least 27 gene mutations and can be affected by age-related macular degeneration (AMD) or diabetic retinopathy³⁴.

LCA is a severe rod-cone dystrophy disease that can lead to blindness shortly after birth. Autosomal recessive inheritance is the main inheritance pattern in patients with LCA, and more than 20 related gene mutations have been identified to date³⁵. RP is an ocular disease that causes the progressive death of photoreceptor cells is thought to be controlled by apoptosis³⁶. The first RP-related gene mutation was reported in 1990, and more than 100 such gene mutations have been identified thus far³⁷. The first general symptom of RP is night blindness, which is followed by a loss of central vision and eventually full blindness³⁸. Different genotypes can result in the same phenotype, and, vice versa, one genotype may result in different phenotypes. Many factors are involved in photoreceptor degeneration. The DNA sequence, transcription, posttranscriptional modifications, translation, and posttranslational modifications are five main elements that can influence the function of the final protein; each element represents a different area of research. However, with the rapid development of epigenetics in recent years, researchers have gradually discovered that retinal degeneration is closely associated with epigenetic regulation³⁹.

Effects of HDACs and HATs on photoreceptor degeneration, underlying mechanisms of action, and potential therapies

Animal models are necessary for the study of retinal degenerative diseases, such as RP and AMD⁴⁰. Cyclic nucleotide phosphodiesterase-6 (PDE6) is a key enzyme that regulates the intracellular levels of cyclic guanosine monophosphate (cGMP). A mutation in PDE6 can lead to cGMP accumulation, which further results in a loss of photoreceptors⁴¹. Two known mutations in the loci of the PDE6β and PDE6c subunits are rd1 (retinal degeneration 1) and cpfl1 (cone photoreceptor function loss 1), which result in an early onset and fast progressive death of rods and cones, respectively^{42, 43}. The pathogenesis of rd1 is diverse, including endogenous molecular stimulation in photoreceptor cells and changes in the microenvironment that cause abnormal cell degeneration and death, such as calcium disorders in rd1 mice⁴⁴. The expression of HDACs in a degenerative retina has not yet been sufficiently investigated. The concentration of Sirt1, which is located in the nucleus, increases with photoreceptor degeneration, resulting in an anti-apoptosis protection of the retina, which is related to the DNA repair mechanism and homeostasis maintenance in rd1 mice⁴⁵.

To reveal the effect and determine the mechanism of action of HDACs on photoreceptor loss and to search for a therapeutic alternative, most studies repress specific HDAC proteins using gene knockdown or pharmacological drugs. The latter can be divided into selective and non-selective categories, depending on the targeting efficiency. TSA, a hydroxamic acid, is a selective HDAC inhibitor, specific for class I and II HDACs⁴⁶. Vorinostat (SAHA), a hydroxamic acid derivative and selective HDAC inhibitor, was the first drug approved by the Food and Drug Administration for applications in cancer treatments⁴⁷. A short-chain fatty acid, valproic acid (VPA), is also a selective HDAC inhibitor and is currently used as an anticonvulsant drug⁴⁸. These three HDAC inhibitors are the most commonly used drugs in therapeutic studies of retinal diseases.

Among six studies of clinical treatment of RP with VPA, in which the time of administration varied from 4 to 12 months and the dosage was 400 or 500 mg/day, only one study, conducted in 2017⁴⁹, showed negative results. In five other studies, the outcomes of the drug treatment in patients with RP were compared with those of the control treatment, as well as with the baseline conditions, and the results showed that VPA treatment improved the visual field and visual sensitivity and delayed vision loss (p<0.05)^50-54. Although VPA had a positive impact on RP, some studies suggested that this treatment was not appropriate for all genotypes, and it was even prohibited in some cases of RP^{55, 56}. Therefore, the confirmation of genetic mutations is required before VPA administration. In addition, the long-term efficacy, dosage, standardization, and safety of VPA should all be considered during a clinical trial. In a cpfl1 retina, treatment with TSA could significantly improve cone survival, as well as the cone migration pattern, while SAHA could prevent rod death in rd10 retinal explant cultures^{34, 57}. HDACs were highly expressed in an rd1 retinal explant culture, and injection of TSA resulted in a significant improvement of rod survival⁵⁸. In the 661W cell line, treatment with TSA resulted in an increased binding of E2F-1 and p53 to the Apaf-1 promoter, which in turn led to increased Apaf-1 expression and subsequent apoptosis of cone cells⁵⁹. Additionally, VPA could protect photoreceptor cells from N-methyl-N-nitrosourea-induced apoptosis, not only through its anti-apoptotic effects but also through its ability to inhibit HSP70 degradation⁶⁰.

The retina is an energy-consuming organ. Metabolic processes, such as glycometabolism and oxidative stress, are related to photoreceptor death, and HDACs play a regulatory role in this process. Acute hypoglycemia can impair human retinal photoreception, cause central vision injury, and eventually lead to blindness⁶¹. SIRT6 can control glucose homeostasis and maintain normal retinal function through the deacetylation of the H3K9 and H3K56 sites of multiple glycometabolic genes, such as GLUT1 and GRM6⁶². SIRTs require NAD⁺ as a cofactor, which makes them highly sensitive to oxidative stress. One process of a pathological change in AMD involves the initiation of SIRT1 activation by hypoxia to enhance the expression of hypoxia-inducible factor 2α, which in turn activates the release of the vascular endothelial growth factor⁶³. HDAC6 exists both in the nucleus and cytoplasm, and in the cytoplasm, it can act on its substrate protein, tubulin. Selective inhibition of HDAC6 with tubastatin A could protect photoreceptors from reactive oxygen species by enhancing the expression of HSP70, HSP25, and peroxiredoxin 1⁶⁴. DNA microarray assays showed that genes related to retinal proliferation and oxidative stress were upregulated in rd1 mice. The exact relationships between these genes and activities of individual HDACs have not yet been elucidated, although one study found an increase in class I and II HDACs before retinal degeneration^{58, 65}. The overexpression of HDAC4 in electroporated rd1 mouse retinas could prolong the photoreceptor survival in a way that might be related to hypoxia-inducible factor 1α activity in the cytoplasm⁶⁶. These results seem to be different from previous data^{34, 57, 67}, which showed that the inhibition of HDACs could protect photoreceptors from degeneration under certain conditions. One explanation may be that HDAC4 plays a neuroprotective role for photoreceptors when it is located in the cytoplasm, which indicates that its substrates are not histone proteins. Moreover, the abovementioned results may indicate that HDACs have multiple functions in photoreceptor degeneration; therefore, more studies are needed to elucidate the mechanisms underlying the role of HDACs in photoreceptor degeneration. A recent study⁶⁸ suggested that a short N-terminal domain of HDAC4 plays a critical role in photoreceptor protection, thus confirming the abovementioned results.

Effective treatment has not been hitherto found for hereditary photoreceptor degeneration⁶⁹, but two potential therapies are being developed. One involves gene therapy, and the other is replacement therapy, which involves the production of new photoreceptor cells from stem cells^{70, 71}. To establish the regulatory role of epigenetic mechanisms, we focused on the process of differentiation of stem cells into photoreceptors. Depending on their origin, stem cells can be classified as exogenous, including mesenchymal, neural, embryonic, and induced pluripotent stem cells, and endogenous, including Müller glia, ciliary epithelial, and retinal pigment epithelial cells⁷². It will be a major focus in the future to determine ways to build and advocate for an ethical embryonic stem cell bank for the benefit of humanity. Recent studies have successfully derived rod photoreceptors from mouse and human embryonic stem cells⁷¹. Müller glia cells in zebrafish can transdifferentiate into photoreceptors, while those in mammals cannot spontaneously regenerate. It is important to note that epigenetic regulation, including histone acetylation, may act as a key stimulation switch in the trans-differentiation of Müller glia cells into photoreceptors ⁷³. The success of photoreceptor regeneration is only the first step in replacement therapy, and successful cell transplantation to recipients remains a great challenge. Scientists are pursuing this goal by studying the right moment for transplantation, repression of the immune reaction after transplantation, and establishment of an appropriate growth microenvironment^74-76. The exact modulatory effects of epigenetic factors, including histone acetylation, on the process of differentiation from stem cells to photoreceptors remain to be explored.

Discussion

With the development of epigenetic approaches, the study of human diseases is no more limited to DNA sequencing. Histone acetylation, an epigenetic modification, mostly occurs at specific Lys residues in the N-terminal alkaline amino acid region of core histones. While HATs acetylate histones, which results in their dissociation from negatively charged DNA, a loosening of the chromatin structure, and the initiation of gene transcription, HDACs deacetylate histones, which results in their tight binding to negatively charged DNA, a dense chromatin packaging, and the suppression of gene transcription. The effect of HATs and HDACs on retinal degeneration has recently gained a significant research interest. In this review, we principally focused on the diseases caused by photoreceptor degeneration in an attempt to reveal the relationships among HATs, HDACs, and photoreceptor differentiation and degeneration.

The effects of HDACs on photoreceptors were the main focus of the studies reviewed, and the association between HATs and photoreceptor degeneration remains to be further explored. Most studies focused on general effects of HDACs on photoreceptor cells, while specific effects of individual HDACs remain to be elucidated. In most cases, studies used pharmacological HDAC inhibition to reveal the effects of HDACs, and the specificity of an HDAC inhibitor should be considered to establish the precise underlying mechanism of HDAC effects on photoreceptor differentiation and degeneration. To activate and repress the transcription of HATs and HDACs, they must form activator or repressor complexes with other proteins, such as transcription factors; therefore, exploring these complexes should be a key research focus in future studies. Moreover, multiple molecules and pathways participate in the differentiation and degeneration of photoreceptor cells and influence their physiological and pathological processes. Therefore, clarifying the roles of HATs and HDACs in photoreceptor differentiation and degeneration and constructing a complete reticular regulation map are necessary for the prevention of and development of treatment options for photoreceptor degeneration. The mechanisms of differentiation, degeneration, and redifferentiation of photoreceptors significantly vary among species. Research on exact differences in these mechanisms among species is another important area of focus to correlate the results obtained in different animal models with those obtained in humans. With the development of epigenetic high-throughput sequencing technology, more breakthroughs will be achieved in the elucidation of the effects of HATs and HDACs on photoreceptors. These enzymes may also represent promising targets for the treatment of ophthalmic diseases caused by photoreceptor degeneration.

Abbreviations

HAT: histone acetyltransferase; HDAC: histone deacetylase; JNK1: c-Jun N-terminal kinase 1; NRL: neural retina leucine zipper; Tip60: HIV Tat-interacting protein; CRX: cone-rod homeobox; TSA: trichostatin A; PNR: photoreceptor cell-specific nuclear receptor; Rb: retinoblastoma protein; RP: retinitis pigmentosa; LCA: Leber congenital amaurosis; AMD: age-related macular degeneration; PDE6: cyclic nucleotide phosphodiesterase-6; cGMP: cyclic guanosine monophosphate; rd1: retinal degeneration 1; cpfl1: cone photoreceptor function loss 1; SAHA: vorinostat; VPA: valproic acid; ONL: outer nuclear layer; OPL: outer plexiform layer; IPL: inner plexiform layer; OTX2: orthodenticle homolog 2; RAX: retinal homeobox gene; Shh: Sonic hedgehog; Ret-CoR: photoreceptor cell-specific nuclear receptor co-repressor.

Acknowledgements

This work was supported by grants from the National Key Research and Development Program (2018YFA0107303) and Initiation Fund of Distinguished Professor, Zhengzhou University (No. 32310180).

Competing Interests

The authors have declared that no competing interest exists.

References

1. Hoshino A, Ratnapriya R, Brooks MJ. et al. Molecular Anatomy of the Developing Human Retina. Dev Cell. 2017;43(6):763-779.e4

2. Hoon M, Okawa H, Della Santina L, Wong RO. Functional architecture of the retina: development and disease. Prog Retin Eye Res. 2014;42:44-84

3. Young RW. Cell differentiation in the retina of the mouse. Anat Rec. 1985;212:199-205

4. Ruzycki PA, Zhang X, Chen S. CRX directs photoreceptor differentiation by accelerating chromatin remodeling at specific target sites. Epigenetics Chromatin. 2018;11(1):42

5. Riesenberg AN, Liu Z, Kopan R, Brown NL. Rbpj cell autonomous regulation of retinal ganglion cell and cone photoreceptor fates in the mouse retina. J Neurosci. 2009;29(41):12865-77

6. Levine EM, Roelink H, Turner J, Reh TA. Sonic hedgehog promotes rod photoreceptor differentiation in mammalian retinal cells in vitro. J Neurosci. 1997;17(16):6277-88

7. Yang F, Ma H, Ding XQ. Thyroid Hormone Signaling in Retinal Development, Survival, and Disease. Vitam Horm. 2018;106:333-349

8. Rodgers HM, Huffman VJ, Voronina VA, Lewandoski M, Mathers PH. The role of the Rx homeobox gene in retinal progenitor proliferation and cell fate specification. Mech Dev. 2018;151:18-29

9. Omori Y, Kubo S, Kon T. et al. Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity. Proc Natl Acad Sci U S A. 2017;114:E8264-8264E8273

10. Ct W, Morris JR. Genes, genetics, and epigenetics: a correspondence. Science. 2001;293:1103-5

11. Portela A, Esteller M. Epigenetic modifications and human disease. Nat Biotechnol. 2010;28:1057-68

12. Shahbazian MD, Grunstein M. Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem. 2007;76:75-100

13. Wang Z, Zang C, Cui K. et al. Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes. Cell. 2009;138:1019-31

14. Egger G, Liang G, Aparicio A, Jones PA. Epigenetics in human disease and prospects for epigenetic therapy. Nature. 2004;429:457-63

15. Sharon Y. Roth JMD, Allis aCD. Histone acetyltransferases. Annu. Rev. Biochem. 2001(70):81-120

16. Grunstein M. Histone acetylation in chromatin structure and transcription. Nature. 1997;389(6649):349-52

17. Giannini G, Cabri W, Fattorusso C, Rodriquez M. Histone deacetylase inhibitors in the treatment of cancer: overview and perspectives. Future Med Chem. 2012;4:1439-60

18. Yang XJ, Seto E. The Rpd3/Hda1 family of lysine deacetylases: from bacteria and yeast to mice and men. Nat Rev Mol Cell Biol. 2008;9:206-18

19. Rouble AN, Storey KB. Characterization of the SIRT family of NAD+-dependent protein deacetylases in the context of a mammalian model of hibernation, the thirteen-lined ground squirrel. Cryobiology. 2015;71:334-43

20. Cho Y, Cavalli V. HDAC signaling in neuronal development and axon regeneration. Curr Opin Neurobiol. 2014;27:118-26

21. Michishita E, Park JY, Burneskis JM. et al. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Mol Biol Cell. 2005;16:4623-35

22. Swaroop A, Kim D, Forrest D. Transcriptional regulation of photoreceptor development and homeostasis in the mammalian retina. Nat Rev Neurosci. 2010;11:563-76

23. Hendrickson A, Bumsted-O'Brien K, Natoli R. et al. Rod photoreceptor differentiation in fetal and infant human retina. Exp Eye Res. 2008;87:415-26

24. Kim JW, Jang SM, Kim CH. et al. Transcriptional activity of neural retina leucine zipper (Nrl) is regulated by c-Jun N-terminal kinase and Tip60 during retina development. Mol Cell Biol. 2012;32:1720-32

25. Hennig AK, Peng GH, Chen S. Transcription coactivators p300 and CBP are necessary for photoreceptor-specific chromatin organization and gene expression. PLoS One. 2013;8:e69721

26. Chen B, Cepko CL. Requirement of histone deacetylase activity for the expression of critical photoreceptor genes. BMC Dev Biol. 2007;7:78

27. Ferreira RC, Popova EY, James J. et al. Histone Deacetylase 1 Is Essential for Rod Photoreceptor Differentiation by Regulating Acetylation at Histone H3 Lysine 9 and Histone H4 Lysine 12 in the Mouse Retina. J Biol Chem. 2017;292:2422-40

28. Ishikawa M, Hasebe M. Cell cycle reentry from the late S phase: implications from stem cell formation in the moss Physcomitrella patens. J Plant Res. 2015;128(3):399-405

29. Matson JP, Cook JG. Cell cycle proliferation decisions: the impact of single cell analyses. FEBS J. 2017;284(3):362-375

30. Stadler JA, Shkumatava A, Norton WH. et al. Histone deacetylase 1 is required for cell cycle exit and differentiation in the zebrafish retina. Dev Dyn. 2005;233:883-9

31. Bienvenu F, Jirawatnotai S, Elias JE. et al. Transcriptional role of cyclin D1 in development revealed by a genetic-proteomic screen. Nature. 2010;463:374-8

32. Brehm A, Miska EA, McCance DJ. et al. Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature. 1998;391:597-601

33. Arango-Gonzalez B, Trifunović D, Sahaboglu A. et al. Identification of a common non-apoptotic cell death mechanism in hereditary retinal degeneration. PLoS One. 2014;9(11):e112142

34. Trifunović D, Arango-Gonzalez B, Comitato A. et al. HDAC inhibition in the cpfl1 mouse protects degenerating cone photoreceptors in vivo. Hum Mol Genet. 2016;25(20):4462-4472

35. Kuribayashi H, Baba Y, Iwagawa T. et al. Roles of Nmnat1 in the survival of retinal progenitors through the regulation of pro-apoptotic gene expression via histone acetylation. Cell Death Dis. 2018;9:891

36. Daiger SP, Sullivan LS, Bowne SJ. Genes and mutations causing retinitis pigmentosa. Clin Genet. 2013;84:132-41

37. Koch SF, Tsai YT, Duong JK. et al. Halting progressive neurodegeneration in advanced retinitis pigmentosa. J Clin Invest. 2015;125:3704-13

38. Hartong DT, Berson EL, Dryja TP. Retinitis pigmentosa. Lancet. 2006;368:1795-809

39. Zhang Q. Retinitis Pigmentosa: Progress and Perspective. Asia Pac J Ophthalmol (Phila). 2016;5:265-71

40. Baehr W, Frederick JM. Naturally occurring animal models with outer retina phenotypes. Vision Res. 2009;49:2636-52

41. Ma EY, Lewis A, Barabas P. et al. Loss of Pde6 reduces cell body Ca(2+) transients within photoreceptors. Cell Death Dis. 2013;4:e797

42. Chang B, Grau T, Dangel S. et al. A homologous genetic basis of the murine cpfl1 mutant and human achromatopsia linked to mutations in the PDE6C gene. Proc Natl Acad Sci U S A. 2009;106(46):19581-6

43. He X, Sun D, Chen S, Xu H. Activation of liver X receptor delayed the retinal degeneration of rd1 mice through modulation of the immunological function of glia. Oncotarget. 2017;8(19):32068-32082

44. Schön C, Paquet-Durand F, Michalakis S. Cav1.4 L-Type Calcium Channels Contribute to Calpain Activation in Degenerating Photoreceptors of rd1 Mice. PLoS One. 2016;11:e0156974

45. Jaliffa C, Ameqrane I, Dansault A. et al. Sirt1 involvement in rd10 mouse retinal degeneration. Invest Ophthalmol Vis Sci. 2009;50:3562-72

46. Rahman MM, Kukita A, Kukita T. et al. Two histone deacetylase inhibitors, trichostatin A and sodium butyrate, suppress differentiation into osteoclasts but not into macrophages. Blood. 2003;101:3451-9

47. Dokmanovic M, Clarke C, Marks PA. Histone deacetylase inhibitors: overview and perspectives. Mol Cancer Res. 2007;5:981-9

48. Zhang H, Dai X, Qi Y. et al. Histone Deacetylases Inhibitors in the Treatment of Retinal Degenerative Diseases: Overview and Perspectives. J Ophthalmol. 2015;2015:250812

49. Totan Y, Güler E, Yüce A, Dervişogulları MS. The adverse effects of valproic acid on visual functions in the treatment of retinitis pigmentosa. Indian J Ophthalmol. 2017;65(10):984-988

50. Clemson CM, Tzekov R, Krebs M, Checchi JM, Bigelow C, Kaushal S. Therapeutic potential of valproic acid for retinitis pigmentosa. Br J Ophthalmol. 2011;95(1):89-93

51. Shanmugam PM, Minija CK, Ramanjulu R, Tekwani P, Saxena M. Effect of short-term oral valproic Acid on vision and visual field in retinitis pigmentosa. Ophthalmol Ther. 2012;1(1):6

52. Kumar A, Midha N, Gogia V, Gupta S, Sehra S, Chohan A. Efficacy of oral valproic acid in patients with retinitis pigmentosa. J Ocul Pharmacol Ther. 2014;30(7):580-6

53. Daly C, Yin J, Kennedy BN. Histone Deacetylase: Therapeutic Targets in Retinal Degeneration. Adv Exp Med Biol. 2016;854:455-61

54. Iraha S, Hirami Y, Ota S. et al. Efficacy of valproic acid for retinitis pigmentosa patients: a pilot study. Clin Ophthalmol. 2016;10:1375-84

55. Arjamaa O, Minn H. Valproic acid treatment may be harmful in non-dominant forms of retinitis pigmentosa. Br J Ophthalmol. 2012;96(8):1153-4

56. Vent-Schmidt R, Wen RH, Zong Z. et al. Opposing Effects of Valproic Acid Treatment Mediated by Histone Deacetylase Inhibitor Activity in Four Transgenic X. laevis Models of Retinitis Pigmentosa. J Neurosci. 2017;37(4):1039-1054

57. Trifunović D, Petridou E, Comitato A, Marigo V, Ueffing M, Paquet-Durand F. Primary Rod and Cone Degeneration Is Prevented by HDAC Inhibition. Adv Exp Med Biol. 2018;1074:367-373

58. Sancho-Pelluz J, Alavi MV, Sahaboglu A. et al. Excessive HDAC activation is critical for neurodegeneration in the rd1 mouse. Cell Death Dis. 2010;1:e24

59. Wallace DM, Cotter TG. Histone deacetylase activity in conjunction with E2F-1 and p53 regulates Apaf-1 expression in 661W cells and the retina. J Neurosci Res. 2009;87:887-905

60. Koriyama Y, Sugitani K, Ogai K, Kato S. Heat shock protein 70 induction by valproic acid delays photoreceptor cell death by N-methyl-N-nitrosourea in mice. J Neurochem. 2014;130:707-19

61. McCrimmon RJ, Deary IJ, Huntly BJ. et al. Visual information processing during controlled hypoglycaemia in humans. Brain. 1996;119( Pt 4):1277-87

62. Silberman DM, Ross K, Sande PH. et al. SIRT6 is required for normal retinal function. PLoS One. 2014;9:e98831

63. Balaiya S, Khetpal V, Chalam KV. Hypoxia initiates sirtuin1-mediated vascular endothelial growth factor activation in choroidal endothelial cells through hypoxia inducible factor-2α. Mol Vis. 2012;18:114-20

64. Leyk J, Daly C, Janssen-Bienhold U. et al. HDAC6 inhibition by tubastatin A is protective against oxidative stress in a photoreceptor cell line and restores visual function in a zebrafish model of inherited blindness. Cell Death Dis. 2017;8:e3028

65. Hackam AS, Strom R, Liu D. et al. Identification of gene expression changes associated with the progression of retinal degeneration in the rd1 mouse. Invest Ophthalmol Vis Sci. 2004;45:2929-42

66. Chen B, Cepko CL. HDAC4 regulates neuronal survival in normal and diseased retinas. Science. 2009;323:256-9

67. Samardzija M, Masarini K, Ueffing M, Trifunović D. HDAC Inhibition Prevents Primary Cone Degeneration Even After the Onset of Degeneration. Adv Exp Med Biol. 2019;1185:383-387

68. Guo X, Wang SB, Xu H. et al. A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa. Nat Commun. 2015;6:8005

69. Ferrari S, Di Iorio E, Barbaro V. et al. Retinitis pigmentosa: genes and disease mechanisms. Curr Genomics. 2011;12:238-49

70. Zhang W, Li L, Su Q. et al. Gene Therapy Using a miniCEP290 Fragment Delays Photoreceptor Degeneration in a Mouse Model of Leber Congenital Amaurosis. Hum Gene Ther. 2018;29:42-50

71. Lu AQ, Barnstable CJ. Generation of Photoreceptor Precursors from Mouse Embryonic Stem Cells. Stem Cell Rev. 2018;14:247-61

72. Ben Mead MB, Ben AS. Stem cell treatment of degenerative eye disease. Stem Cell Res. 2015;14:243-57

73. Jorstad NL, Wilken MS, Grimes WN. et al. Stimulation of functional neuronal regeneration from Müller glia in adult mice. Nature. 2017;548:103-7

74. Zhu J, Cifuentes H, Reynolds J, Lamba DA. Immunosuppression via Loss of IL2rγ Enhances Long-Term Functional Integration of hESC-Derived Photoreceptors in the Mouse Retina. Cell Stem Cell. 2017;20:374-84.e5

75. MacLaren RE, Pearson RA, MacNeil A. et al. Retinal repair by transplantation of photoreceptor precursors. Nature. 2006;444:203-7

76. Zou T, Gao L, Zeng Y. et al. Organoid-derived C-Kit+/SSEA4- human retinal progenitor cells promote a protective retinal microenvironment during transplantation in rodents. Nat Commun. 2019;10:1205

77. Suka N, Suka Y, Carmen AA, Wu J, Grunstein M. Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin. Mol Cell. 2001;8(2):473-9

78. Grant PA, Eberharter A, John S, Cook RG, Turner BM, Workman JL. Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem. 1999;274(9):5895-900

79. Bird AW, Yu DY, Pray-Grant MG. et al. Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair. Nature. 2002;419(6905):411-5

80. Morris SA, Rao B, Garcia BA. et al. Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification. J Biol Chem. 2007;282(10):7632-40

81. Schiltz RL, Mizzen CA, Vassilev A, Cook RG, Allis CD, Nakatani Y. Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates. J Biol Chem. 1999;274(3):1189-92

82. Kuo MH, Brownell JE, Sobel RE. et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature. 1996;383(6597):269-72

83. Parthun MR, Widom J, Gottschling DE. The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism. Cell. 1996;87(1):85-94

84. Ye J, Ai X, Eugeni EE. et al. Histone H4 lysine 91 acetylation a core domain modification associated with chromatin assembly. Mol Cell. 2005;18(1):123-30

85. Winkler GS, Kristjuhan A, Erdjument-Bromage H, Tempst P, Svejstrup JQ. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proc Natl Acad Sci U S A. 2002;99(6):3517-22

86. Angus-Hill ML, Dutnall RN, Tafrov ST, Sternglanz R, Ramakrishnan V. Crystal structure of the histone acetyltransferase Hpa2: A tetrameric member of the Gcn5-related N-acetyltransferase superfamily. J Mol Biol. 1999;294(5):1311-25

87. Sutton A, Shia WJ, Band D. et al. Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex. J Biol Chem. 2003;278(19):16887-92

88. Hilfiker A, Hilfiker-Kleiner D, Pannuti A, Lucchesi JC. mof, a putative acetyl transferase gene related to the Tip60 and MOZ human genes and to the SAS genes of yeast, is required for dosage compensation in Drosophila. EMBO J. 1997;16(8):2054-60

89. Howe L, Auston D, Grant P. et al. Histone H3 specific acetyltransferases are essential for cell cycle progression. Genes Dev. 2001;15(23):3144-54

90. Kimura A, Horikoshi M. Tip60 acetylates six lysines of a specific class in core histones in vitro. Genes Cells. 1998;3(12):789-800

91. Daujat S, Bauer UM, Shah V, Turner B, Berger S, Kouzarides T. Crosstalk between CARM1 methylation and CBP acetylation on histone H3. Curr Biol. 2002;12(24):2090-7

92. Tie F, Banerjee R, Stratton CA. et al. CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing. Development. 2009;136(18):3131-41

93. Zhang B, Day DS, Ho JW. et al. A dynamic H3K27ac signature identifies VEGFA-stimulated endothelial enhancers and requires EP300 activity. Genome Res. 2013;23(6):917-27

94. Haberland M, Montgomery RL, Olson EN. The many roles of histone deacetylases in development and physiology: implications for disease and therapy. Nat Rev Genet. 2009;10:32-42

95. Morris MJ, Monteggia LM. Unique functional roles for class I and class II histone deacetylases in central nervous system development and function. Int J Dev Neurosci. 2013;31:370-81

Author contact

Corresponding authors: ghpedu.cn; panghtoyezzedu.cn; Tel.: +86-0371-6778-1855 (G.-H.P.); Fax.: +86-0371-6778-1959 (G.-H.P.)

Received 2019-12-17
Accepted 2020-5-10
Published 2020-5-23

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