Int J Med Sci 2020; 17(5):599-608. doi:10.7150/ijms.39106 This issue

Research Paper

LRCH1 suppresses migration of CD4+ T cells and refers to disease activity in ulcerative colitis

Yibo Wang1, Hairong Zhang1, Heng He1, Kuankuan Ai1, Wei Yu1, Xiao Xiao1, Yufen Qin1, Lingming Zhang1, Huabao Xiong2✉, Guangxi Zhou1✉

1. Department of Gastroenterology, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong 272000, P.R. China.
2. Institute of Immunology and Molecular Medicine, Jining Medical University, Jining, Shandong 272000, P.R. China.

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Citation:
Wang Y, Zhang H, He H, Ai K, Yu W, Xiao X, Qin Y, Zhang L, Xiong H, Zhou G. LRCH1 suppresses migration of CD4+ T cells and refers to disease activity in ulcerative colitis. Int J Med Sci 2020; 17(5):599-608. doi:10.7150/ijms.39106. Available from https://www.medsci.org/v17p0599.htm

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Abstract

Background: Ulcerative colitis (UC) is a chronically remittent and progressive inflammatory disorder. LRCH1 is reported to be involved in the immune-regulation of several diseases. However, the exact roles of LRCH1 in UC are still obscure.

Materials and Methods: LRCH1 expression was analyzed in the inflamed mucosa and peripheral blood mononuclear cells (PBMCs) from patients with UC by quantitative RT-PCR and immunohistochemistry. Peripheral blood CD4+ T cells were transfected with lentivirus-expressing LRCH1 (LV-LRCH1) or LV-sh-LRCH1, and cytokine expression was determined by using flow cytometry, quantitative RT-PCR and ELISA. Transfected CD4+ T cells were harvested to examine the capacity of chemotaxis using Transwell plate.

Results: LRCH1 expression was highly decreased in colonic mucosa and PBMCs from patients with A-UC, and negatively correlated with disease activity. Up or down regulation of LRCH1 did not affect the differentiation of CD4+ T cells, and the related cytokines expression. Moreover, LRCH1 inhibited migratory capacity of CD4+ T cells toward CXCL12 by PKCα.

Conclusion: LRCH1 plays an important role in the pathogenesis of UC, possibly through modulating the migration of CD4+ T cells. Therefore, targeting LRCH1 might serve as a novel therapeutic approach in the management of UC.

Keywords: LRCH1, ulcerative colitis, CD4+ T cells, migration