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Int J Med Sci 2020; 17(2):242-254. doi:10.7150/ijms.38657 This issue Cite

Research Paper

Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene

Milena Ściskalska1✉, Monika Ołdakowska1, Grzegorz Marek2, Halina Milnerowicz1✉

1. Department of Biomedical and Environmental Analyses, Faculty of Pharmacy, Wroclaw Medical University, Wroclaw 50-556, Poland
2. Second Department of General and Oncological Surgery, Wroclaw Medical University, Wroclaw 50-556, Poland

✉ Corresponding authors: Milena Ściskalska, PhD, e-mail: milena.sciskalskawroc.pl, ORCID ID: 0000-0001-8976-6683 or Halina Milnerowicz, Professor, PhD., ScD., E-mail: halina.milnerowiczwroc.pl, ORCID ID: 0000-0002-0772-9852. Departmen More

Citation:
Ściskalska M, Ołdakowska M, Marek G, Milnerowicz H. Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene. Int J Med Sci 2020; 17(2):242-254. doi:10.7150/ijms.38657. https://www.medsci.org/v17p0242.htm
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Abstract

Objectives: The study was aimed to assess γ‑glutamyltransferase (GGT) activity and concentration as a marker of oxidative stress induced by exposure to tobacco smoke in acute pancreatitis (AP) course. Examination of the relationship between GGT activity/concentration and single-nucleotide polymorphism (SNP rs5751901 and rs2236626) in GGT1 gene was performed.

Subjects and methods: We examined SNPs in 38 AP patients and 51 healthy subjects by PCR-RFLP methods. GGT concentration in blood was measured with the use of the ELISA method; GGT activity and GSH concentration were measured by the Szasz and Patterson methods, respectively.

Results: In the non-AP smokers group with TC genotype for SNPrs5751901 an increased blood GGT activity compared to smokers with CC genotypes was shown. In the course of AP was observed an elevated GGT activity and the value of GGT activity/GGT concentration ratio in smokers compared to non-smokers, in AP patients with TC genotypes and CC genotypes, respectively, for both SNP: rs5751901 and rs2236626. In the group of smoking AP patients with the CC and TC genotypes in rs5751901 locus and CC and TT genotypes in rs2236626 locus a decreases in GSH concentration during hospitalization were noted.

Conclusions: SNP rs5751901 and rs2236626 cause changes in GGT activity. Smoking in the AP course contributes to increased GGT activity and excessive GSH use up in patients with TC and CC genotypes for both SNPs. Exposure to smoke xenobiotics enhances (3-fold) the risk of AP occurrence in individuals with TC genotypes for SNP rs5751901.

Keywords: rs5751901, rs2236626, smoking, polymorphism, γ-glutamyltransferase, acute pancreatitis

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APA
Ściskalska, M., Ołdakowska, M., Marek, G., Milnerowicz, H. (2020). Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene. International Journal of Medical Sciences, 17(2), 242-254. https://doi.org/10.7150/ijms.38657.

ACS
Ściskalska, M.; Ołdakowska, M.; Marek, G.; Milnerowicz, H. Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene. Int. J. Med. Sci. 2020, 17 (2), 242-254. DOI: 10.7150/ijms.38657.

NLM
Ściskalska M, Ołdakowska M, Marek G, Milnerowicz H. Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene. Int J Med Sci 2020; 17(2):242-254. doi:10.7150/ijms.38657. https://www.medsci.org/v17p0242.htm

CSE
Ściskalska M, Ołdakowska M, Marek G, Milnerowicz H. 2020. Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene. Int J Med Sci. 17(2):242-254.

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