Int J Med Sci 2019; 16(8):1072-1077. doi:10.7150/ijms.34440 This issue


Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells

Qin Zhu1*, Hong Sun2*, Dongmei Yang1, Sean Tighe3, Yongsong Liu4, Yingting Zhu3✉, Min Hu1✉

1. Department of Ophthalmology, The Second People's Hospital of Yunnan Province (Fourth Affiliated Hospital of Kunming Medical University); Yunnan Eye Institute; Key Laboratory of Yunnan Province for the Prevention and Treatment of ophthalmology (2017DG008); Provincial Innovation Team for Cataract and Ocular Fundus Disease (2017HC010); Expert Workstation of Yao Ke (2017IC064), Kunming 650021, China
2. Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China
3. Tissue Tech, Inc., Ocular Surface Center, and Ocular Surface Research & Education Foundation, Miami, FL, 33173 USA
4. Department of Ophthalmology, Yan' An Hospital of Kunming City, Kunming, 650051, China
*The first two authors contribute equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution License ( See for full terms and conditions.
Zhu Q, Sun H, Yang D, Tighe S, Liu Y, Zhu Y, Hu M. Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells. Int J Med Sci 2019; 16(8):1072-1077. doi:10.7150/ijms.34440. Available from

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Corneal endothelial tissue engineering aims to find solutions for blindness due to endothelial dysfunction. A suitable combination of endothelial cells, substrates and environmental cues should be deployed for engineering functional endothelial tissues. This manuscript reviews up-to-date topics of corneal endothelial tissue engineering with special emphasis on biomaterial substrates and their properties, efficacy, and mechanisms of supporting functional endothelial cells in vitro.

Keywords: substrate, tissue engineering, endothelium, collagen, integrin, focal adhesion kinase, leukemia inhibitory factor