Int J Med Sci 2019; 16(2):231-240. doi:10.7150/ijms.28811 This issue

Research Paper

In Vitro Anti-proliferative and Anti-invasive Effect of Polysaccharide-rich Extracts from Trametes Versicolor and Grifola Frondosa in Colon Cancer Cells

Daniel Roca-Lema1*, Olaia Martinez-Iglesias1*, Catalina Fernández de Ana Portela2, Arturo Rodríguez-Blanco2, Manuel Valladares-Ayerbes3, Andrea Díaz-Díaz1, Alba Casas-Pais1, Cecilia Prego4, Angélica Figueroa1✉

1. Epithelial Plasticity and Metastasis Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas. Universidade da Coruña (UDC).
2. Hifas da Terra SL, Pontevedra, Spain.
3. Department of Medical Oncology, Hospital Universitario Reina Sofía, Córdoba, Spain.
4. CZ Veterinaria SA, Pontevedra, Spain.
*These authors contributed equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license ( See for full terms and conditions.
Roca-Lema D, Martinez-Iglesias O, Fernández de Ana Portela C, Rodríguez-Blanco A, Valladares-Ayerbes M, Díaz-Díaz A, Casas-Pais A, Prego C, Figueroa A. In Vitro Anti-proliferative and Anti-invasive Effect of Polysaccharide-rich Extracts from Trametes Versicolor and Grifola Frondosa in Colon Cancer Cells. Int J Med Sci 2019; 16(2):231-240. doi:10.7150/ijms.28811. Available from

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Colorectal cancer (CRC) is one of leading causes of mortality in western countries and novel treatment strategies are required. The medicinal application of mushrooms has been used in traditional medicine in many oriental countries. Polysaccharide-rich extracts obtained from certain medicinal mushroom species have shown antitumor effects in different experimental models. In the present study, we have developed polysaccharide-rich extracts from Trametes versicolor (TV) and Grifola frondosa (GF) fruit bodies. We aim to evaluate the anticancer effects of these polysaccharide-rich extracts in LoVo and HT-29 human colon cancer cells. The in vitro effects were determined by cytotoxicity assay, proliferation assay, wound healing assay and invasion assay. Moreover, the effect on anchorage independent-cell growth was also determined. Our results showed that TV and GF extracts did inhibit human colon cell proliferation and induce cytotoxicity. Furthermore, both fungal extracts significantly inhibited oncogenic potential, cell migration and invasion in colon cancer cells. In addition, extracts induce a more epithelial phenotype, observed by phase contrast images, together with an increase expression of the E-cadherin epithelial marker, detected by western-blotting analyses. Moreover, by using gelatin zymography assays, it was detected a decrease of MMP-2 enzyme activity, a crucial metalloproteinase important for the degradation of the extracellular matrix. Finally, the combination of the extracts with one the most clinical used agents for colorectal cancer, 5-fluorouracil, increases cell cytotoxicity. Taken together our results underscore a potential antitumor effect of polysaccharide-rich extracts obtained from TV and GF in human colon cancer cells lines. These finding may contribute to the reported health effects of fungal extracts.

Keywords: Colon cancer, invasion, proliferation, Fugal extracts