Int J Med Sci 2018; 15(11):1251-1259. doi:10.7150/ijms.24170 This issue

Research Paper

Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice

Sen-Wei Tsai1,2, Yi-Ju Hsu3, Mon-Chien Lee3, Hao-En Huang4, Chi-Chang Huang3,5,✉, Yu-Tang Tung5,✉

1. Department of Physical Medicine and Rehabilitation, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung City 427, Taiwan.
2. Department of Physical Medicine and Rehabilitation, School of Medicine, Tzu Chi University, Hualien 970, Taiwan.
3. Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
4. Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan
5. Graduate Institute of Metabolism and Obesity Sciences, Taipei Medical University, Taipei 11031, Taiwan.

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Tsai SW, Hsu YJ, Lee MC, Huang HE, Huang CC, Tung YT. Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice. Int J Med Sci 2018; 15(11):1251-1259. doi:10.7150/ijms.24170. Available from

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Current treatment options for muscle injuries remain suboptimal and often result in delayed/incomplete recovery of damaged muscles. In this study, the effects of dextrose prolotherapy on inflammation and regeneration of skeletal muscles after a contusion injury were investigated. Mice were separated into five groups, including a normal control (NC), post-injury with no treatment (mass-drop injury, MDI), post-injury with 10% dextrose (MDI + 10% dextrose), post-injury with 20% dextrose (MDI + 20% dextrose), and post-injury with 30% dextrose (MDI + 30% dextrose). The gastrocnemius muscles of the mice were subjected to an MDI, and muscle samples were collected at 7 days post-injury. Results showed the serum creatine kinase (CK), blood urea nitrogen (BUN), creatinine (CREA), and low-density lipoprotein (LDH) of the MDI-alone group were significantly higher than those of the normal control group (p<0.05). However, levels of serum CK, BUN, CREA, and lactate dehydrogenase (LDH) significantly decreased with different concentrations of dextrose. In addition, dextrose suppressed the macrophage response (F4/80 protein decreased) and promoted muscle satellite cell regeneration (desmin protein increased). In conclusion, dextrose prolotherapy can effectively help repair muscles; therefore, it may be one of the methods for clinically treating muscle injuries.

Keywords: dextrose, prolotherapy, contusion, mass-drop injury (MDI), diclofenac (DCF).