Int J Med Sci 2014; 11(10):1065-1072. doi:10.7150/ijms.8473 This issue

Research Paper

Bone Morphogenetic Protein-9 Induces PDLSCs Osteogenic Differentiation through the ERK and p38 Signal Pathways

Guo Ye1,2, Conghua Li1,2, Xuerong Xiang1,2, Chu Chen3, Ruyi Zhang3, Xia Yang1,2, Xuesong Yu1,2, Jinhua Wang1,2, Lan Wang1,2, Qiong Shi3✉, Yaguang Weng3✉

1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China
2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China
3. Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine Diagnostics, Chongqing Medical University, Chongqing 400016, China.

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Ye G, Li C, Xiang X, Chen C, Zhang R, Yang X, Yu X, Wang J, Wang L, Shi Q, Weng Y. Bone Morphogenetic Protein-9 Induces PDLSCs Osteogenic Differentiation through the ERK and p38 Signal Pathways. Int J Med Sci 2014; 11(10):1065-1072. doi:10.7150/ijms.8473. Available from

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Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been found to have potent osteogenic activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected with recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of osteogenic markers such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) as well as mineralization ability were measured. The results showed that BMP9 promoted bone formation of PDLSCs. In other experiments, SB203580 and PD98059, which are inhibitors of p38 and ERK1/2, respectively, were used to determine if these kinases are involved in the osteogenic differentiation process. The resulting protein expression profiles and osteogenic markers of PDLSCs revealed that the mitogen-activated protein kinase (MAPK) signaling pathway might play an important role in the process of BMP9-induced osteogenic differentiation of PDLSCs.

Keywords: Periodontal ligament stem cells, BMP9, MAPK, Osteogenesis.