Int J Med Sci 2012; 9(5):339-352. doi:10.7150/ijms.4414 This issue

Research Paper

BioShuttle Mobility in Living Cells Studied with High-Resolution FCS & CLSM Methodologies

Klaus Braun1✉, Marcel Beining2, Manfred Wiessler1, Twan Lammers3, Rüdiger Pipkorn4, Ute Hennrich1, Kiyoshi Nokihara5, Wolfhard Semmler1, Jürgen Debus6, Waldemar Waldeck2

1. German Cancer Research Center, Dept. of Imaging and Radiooncology, INF 280, D-69120 Heidelberg, Germany.
2. German Cancer Research Center, Central Peptide Synthesis Unit, INF 580, D-69120 Heidelberg, Germany.
3. Utrecht University, Dept. of Pharmaceutics, Sorbonnelaan 16, NL-3584 Utrecht, The Nederlands.
4. German Cancer Research Center, Division of Biophysics of Macromolecules, INF 580, D-69120 Heidelberg, Germany.
5. HiPep Laboratories, Nakatsukasa-cho, 486-46, Kamigyo-ku, Kyoto 602-8158, Japan.
6. Heidelberg University Hospital, Dept. of Radiation Oncology, INF 400, D-69120 Heidelberg, Germany.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See for full terms and conditions.
Braun K, Beining M, Wiessler M, Lammers T, Pipkorn R, Hennrich U, Nokihara K, Semmler W, Debus J, Waldeck W. BioShuttle Mobility in Living Cells Studied with High-Resolution FCS & CLSM Methodologies. Int J Med Sci 2012; 9(5):339-352. doi:10.7150/ijms.4414. Available from

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With the increase in molecular diagnostics and patient-specific therapeutic approaches, the delivery and targeting of imaging molecules and pharmacologically active agents gain increasing importance. The ideal delivery system does not exist yet. The realization of two features is indispensable: first, a locally high concentration of target-specific diagnostic and therapeutic molecules; second, the broad development of effective and safe carrier systems. Here we characterize the transport properties of the peptide-based BioShuttle transporter using FFM and CLSM methods. The modular design of BioShuttle-based formulations results in a multi-faceted field of applications, also as a theranostic tool.

Keywords: Drug Delivery, BioShuttle, Confocal Laser Scanning Microscopy-CLSM, Fluorescence Correlation Spectroscopy-FCS, Fluorescence Fluctuation Microscopy-FFM, Ligation chemistry, Non-Viral Carrier Systems, Spinning Disc Microscopy-SDM.