Int J Med Sci 2011; 8(5):353-361. doi:10.7150/ijms.8.353 This issue
1. Department of Anaesthesia and Intensive Care, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
2. Department of Anesthesiology, Shanghai Pneumology Hospital, Tongji University School of Medicine, Shanghai, China.
3. Department of Cardiothoracic surgery, Changhai Hospital, Second Military Medical University, Shanghai, China.
4. Organ Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai, China.
* The first two authors contributed equally to this work.
Objective: To investigate whether emulsified isoflurane preconditioning could reduce lung injury induced by hepatic I/R in rats and its mechanism.
Materials and methods: 32 pentobarbital-anesthetized Sprague-Dawley rats were equally randomized into four groups: laparotomy group (Sham group), hepatic I/R and normal saline infusion group (I/R+S group), I/R and lipid vehicle infusion (I/R+V group), or I/R and 8% emulsified isoflurane infusion (I/R+E group) at the rate of 8 ml·kg-1·h-1 for 30 min. Blood supply of the hepatic artery and portal vein to the left and the median liver lobes was occluded for 90 min after 30-min washout time. Reperfusion was allowed to proceed for 4 h before sacrifice of the animals. Lung injury was observed histologically. Neutrophil infiltration and TNF-α concentration in serum and lung were measured. Changes of wet-to-dry weight ratios in lung tissue, ICAM-1 expression and NF-κB activity in lung after hepatic I/R were determined.
Results: Compared with I/R+S or I/R+V group, emulsified isoflurane preconditioning reduced hepatic I/R-induced lung histologic injury and inhibited the increase of myeloperoxidase (MPO) activity in the lung tissue markedly (5.5±1.37 and 5.22±1.33 vs 3.81±1.62 U/g, P<0.05). In addition, both serum and lung tissue TNF-α levels were reduced in I/R+E group (104.58±31.40 and 94.60±22.23 vs 72.44±17.28 pg/ml, P<0.05; 393.51±88.22 and 405.46±102.87 vs 292.62±74.56 pg/ml, P<0.01). Emulsified isoflurane preconditioning also inhibited the increase of ICAM-1 expression (0.79±0.17 and 0.84±0.24 vs 0.62±0.21, P<0.05) and NF-κB translocation (4.93±0.48 and 4.76±0.57 vs 4.01±0.86, P<0.05) in the lung tissue markedly.
Conclusions: Emulsified isoflurane preconditioning markedly attenuated hepatic I/R-induced lung injury in rats, which may be hopefully applied to the clinical treatment of organ injury caused by hepatic surgery, transplantation or hemorrhagic shock.
Keywords: emulsified isoflurane, inflammation, intercellular adhesion molecule-1, neutrophils, nuclear factor-κB, rats, tumor necrosis factor-α
Ischemia/reperfusion (IR) injury represents a complex series of events, including release of reactive oxygen species, nitric oxide imbalance, cytokine cascades, neutrophil accumulation and cell death, resulting in cellular and tissue damage1. Hepatic I/R injury, which can been seen in various clinical settings such as liver transplantation, hepatectomy, and hemorrhagic shock, may lead to local and remote organ damage2, yet the precise pathogenesis is not fully defined. Massive accumulation of neutrophils in the lung, the development of interstitial pulmonary edema and increased expression of proinflammatory mediators are major features of lung injury induced by hepatic I/R.
Various methods, including pharmacological treatment, gene therapy and ischemia preconditioning, have been applied to ameliorate hepatic I/R injury, with inspiring results. In 1986, Murry et al3 demonstrated for the first time that intermittent episodes of ischemia had a protective effect on the myocardium that was later subjected to a sustained bout of ischemia. A characteristic of ischemic preconditioning is a cross-tolerance phenomenon. The efficacy of anesthetic preconditioning was first described in 1997 with isoflurane in animals4,5, and later confirmed by several studies in the brain6, kidney7 and liver8. Inhaled isoflurane preconditioning was also shown to reduce acute lung injury and inflammation induced by endotoxin9,10 or I/R11.
Emulsified isoflurane has been widely studied in recent years, because it was found to eliminate the need for specific ventilatory circuits, provide rapid anesthetic induction and recovery, have remarkable hemodynamic stability12 and reduce environmental pollution and tissue toxicity. Rao et al13 demonstrated that emulsified isoflurane had a myocardial protective effect on I/R injury similar to that of inhaled isoflurane. We therefore hypothesized that emulsified isoflurane preconditioning might also be able to inhibit inflammation reaction and reduce lung injury induced by hepatic I/R in rats.
Inbred male Sprague-Dawley rats weighing 200-250 g (Experimental Animal Center of the Second Military Medical University, Shanghai, China) were maintained in laminar flow cages in a specific pathogen free animal facility, and allowed free access to standard laboratory chow and water before experiments. This study was approved by the animal care committee at the Second Military Medical University and all procedures in this experiment were performed according to the Guide for the Care and Use of Laboratory Animals.
A model of segmental (70%) hepatic ischemia was used as previously described14,15. Rats were anesthetized intraperitoneally with pentobarbital (40 mg/kg). Body temperature was monitored by a rectal probe and maintained at around 37℃ by a heating lamp. The right carotid artery was cannulated for arterial blood monitoring and blood-gas analysis, and the right jugular vein was cannulated for drug infusion and blood sampling. A midline laparotomy was performed, and an atraumatic clip was applied to interrupt the arterial and portal venous blood supply to the left and median lobes of the liver. The clip was removed 90 min after partial hepatic ischemia to initiate hepatic reperfusion. Sham control rats underwent the same protocol without vascular occlusion. Oxygen was not given during the surgery and throughout the experimental period. Rats were killed after 4-h reperfusion, and lung tissues and blood samples were collected for analysis.
The 8% emulsified isoflurane (v/v) manufactured by Huarui Pharmacy, Ltd (Wuxi, China) according to the procedures described previously16,17, was kindly bestowed by Prof. Jin Liu from the Laboratory of Anesthesiology and Critical Care Medicine, West China Hospital, Sichuan University (Chengdu, China). Briefly, 1.6 mL liquid isoflurane and 18.4 mL 30% Intralipid® (fat emulsion injection, Sino-Swed Pharmaceutical Corp. LTD, China) was mixed in a 20-mL glass ampoule and sealed using an alcohol blowtorch. The ampoule was then vigorously shaken on a vibrator for 15 min to solubilize isoflurane into a lipid emulsion. The emulsified isoflurane ampoule was opened just before use and the residual drug was discarded. Before this experiment, the stability of 8% emulsified isoflurane was investigated by gas chromatography. There was no change in isoflurane concentration nor were lipid droplets found during 6 months of storage at room temperature.
Group 1. Sham (n=8): animals were subjected to anesthesia and laparotomy.
Group 2. I/R+S (n=8): animals were infused with normal saline through the right external jugular vein at the rate of 8 ml·kg-1·h-1 for 30 min, and then subjected to 70% hepatic ischemia for 90 min, followed by 4-h reperfusion.
Group 3. I/R + V (n=8): animals were infused with lipid vehicle (Intralipid®, 30%) through the right external jugular vein at the rate of 8 ml·kg-1·h-1 for 30 min, followed by a 30-min wash-out period before I/R.
Group 4. I/R + E (n=8): animals were infused with emulsified isoflurane through the right external jugular vein at the rate of 8 ml·kg-1·h-1 for 30 min as Rao described13, followed by a 30-min wash-out period before I/R.
Before sacrifice of the animals, arterial blood was sampled from the right carotid artery for blood gas analysis with a blood-gas analyzer (GEM Premier 3000, Instrumentation Laboratory, USA).
The middle lobe of the right lung was excised for histopathology. Samples were fixed in 10% neutral buffered formalin, paraffin embedded, sliced into 5-µm sections, stained with hematoxylin-eosin (H&E) according to standard procedures, and evaluated by light-microscopic examination.
The extent of lung edema was measured by tissue wet to dry weight ratios. The lower lobe of the right lung from each animal was harvested, blotted dry, weighed, incubated at 60℃ overnight and reweighed18. The wet to dry weight ratio was calculated by dividing the wet by the dry weight.
Myeloperoxidase (MPO), a marker of pulmonary neutrophil accumulation and activation, was determined by a modified method of Welborn et al19. Briefly, frozen lung sample (200mg) was homogenized in 0.01 M KH2PO4 at a ratio of 1:10 weight for volume. The pellets were resuspended in 0.5 mL of C-TAB (cetyltrimethylammoniumbromide) buffer. The samples were homogenized, sonicated for 45 s, and subjected to one freeze-thaw cycle. MPO was assayed in the supernatant with the H2O2-dependent oxidation of 3,3',5,5'-tetramethylbenzidine. Absorbance was read at 650 nm and compared with a linear standard curve with sensitivity to 0.008 U. Values were then divided by the wet weight of the lung tissue.
Frozen lung tissue was homogenized in 10 volumes of 50 mmol/L phosphate buffer (pH 6.0). After centrifugation at 4,000g, the supernatant was frozen at -20℃ and saved for measurement of TNF-α level. 2 ml blood obtained from the right jugular vein was centrifuged at 3,000g to get serum, which was saved at -20℃ for measurement of TNF-α levels. Lung tissue and serum TNF-α levels were measured using a commercial rat TNF-α ELISA kit (R&D Systems, USA).
ICAM-1 mRNA from frozen lung tissues was measured using semi-quantitative RT-PCR. Total RNA was extracted from the tissue sample using the Trizol reagent (Invitrogen, Life Technologies) according to the manufacturer's protocol. The RNA concentration was determined by ultraviolet light absorbance at a wavelength of 260nm. The first-strand complementary DNA (cDNA) was synthesized using oligo-dT primer and the AMV reverse transcriptase. The cDNA products were amplified in 50μl reaction volume containing 50 pmol of each primer, 1μl of the cDNA reaction mix, 5μl Buffer (10 mmol/L), 1μl of each dNTP (10mmol/L), and 3 units of Taq DNA polymerase (GIBCO Life Technologies). After 5-min initial melting at 95℃, the mixture was amplified for a total of 30 cycles with a three-step cycle process that began with melting at 95℃ for 45 s, annealing at 60℃ for 30 s, and extension at 72℃ for 45 s. The final cycle was followed by 5-min soaking at 72℃. The nucleotide sequences of the PCR primers were 5'- CTTCAAGCTGAGCGACATTGG -3' (forward) and 5'- AGCATGAGAAATTGGCTCCGT -3' (reverse) for ICAM-1 and 5'- ACCACAGTCCATGCCATCAC -3' (forward) and 5'- TCCACCACCCTGTTGCTGTA -3' (reverse) for GAPDH. The expected size of the amplified cDNA fragments of ICAM-1 and GAPDH was 326 and 452 bp, respectively. Ten microliters of each RT-PCR were electrophoresed in a 1.5% agarose gel and stained with ethidium bromide. The intensity of each ICAM-1 mRNA band was quantified by densitometry using a gel documentation and analysis system and normalized to values for GAPDH.
Nuclear proteins were prepared from lung tissues according to the modified protocols of previously studies20,21. Briefly, frozen liver tissues were homogenized in cold buffer A containing 10mM HEPES-KOH, 1.5mM MgCl2, 10mM KCl, 1mM phenylmenthysulfonylfluoride (PMSF), 1mM dithiothreitol(DTT) and 0.1mM EDTA. The homogenate was centrifuged at 450g for 1 min at 4℃. The supernatant was collected and incubated on ice for 30 min, vortexed for 30 s after addition of 10% NP-40, then centrifuged at 5,000g for 3 min at 4℃. The pellet (nuclei) was resuspended in cold buffer B containing 20mM HEPES-KOH, 25% glycerol, 420mM NaCl, 1.5mM MgCl2, 1mM PMSF, 1mM DTT, and 0.1mM EDTA, and incubated for 30 min with intermittent stirring. The suspension was centrifuged at 15,000g for 10min at 4℃, and the protein concentration was determined by Coomassie blue dye-binding assay. An equal amount of protein was mixed with the sample buffer, separated by 10% SDS-PAGE, and transferred to nitrocellulose membranes. The membrane was blocked for 1 h at room temperature with blocking solution (3% nonfat milk in Tris buffered saline with Tween 20). Blots were then incubated overnight at 4℃ with mouse monoclonal anti-NF-κB p65 antibody (Santa Cruz Biotechnology, 1:500), washed three times, and incubated with a horseradish peroxidase-labeled secondary antibody for 1 h at room temperature. Immunoreactive proteins were visualized with the use of enhanced chemiluminescence detection (Pierce, USA). The protein band density was quantified by densitometric techniques and expressed as mean relative densitometric units.
Data were expressed as mean ± SD. The statistical analysis was carried out using SPSS 13.0 for Windows. All data were analyzed by ANOVA, followed by the Student-Newman-Keuls test. P<0.05 was considered statistically significant.
Compared with sham group, the IR+S and IR+V group had significantly lower PaO2 and higher PaCO2 (P < 0.05). Preconditioning with emulsified isoflurane improved pulmonary function, as indicated with higher PaO2 and lower PaCO2, while pH, HCO2- and SpO2 in IR+S and IR+V groups were lower than those in sham and IR+E groups, but the difference was not statistically significant (P>0.05, Table 1).
Arterial blood gas analysis
Data are expressed as mean ± SD. a p <0.05 vs I/R+S group or I/R+V group.
The effects of emulsified isoflurane preconditioning on the histopathological changes of the lungs in rats with hepatic I/R are shown in Figure 1.
Morphologic changes of the lung. A, sham group: No histologic alteration was observed. B, IR+S group: the inflammatory process was observed as represented by infiltration of leukocytes into interstitial and alveolar spaces, edema and partial destruction of the pulmonary architecture. C, IR+V group: Similar to IR+S group D, IR+E group: Lung pathology was attenuated to a great extent. Original magnification: ×400.
Blind analysis was performed on all samples to evaluate pulmonary architecture, tissue edema formation and infiltration of the inflammatory cells. The results were classified into four grades where Grade 1 represented normal histopathology; Grade 2 mild infiltration of neutrophilic leukocytes; Grade 3 moderate infiltration of neutrophilic leukocytes with perivascular edema formation and partial destruction of the pulmonary architecture and Grade 4 dense infiltration of neutrophilic leukocyte associated with abcess formation and complete destruction of the pulmonary architecture. Pulmonary histology was normal in sham group (Grade 1, Fig. 1A). In contrast, morphological study showed that the lung tissues in the saline treated and fat vehicle treated groups were severely damaged 90 min after hepatic ischemia and 4 h after reperfusion, as represented by marked infiltration of leukocytes into interstitial and alveolar spaces, edema and partial destruction of the pulmonary architecture (Grade 3, Fig. 1B & 1C), while only moderate lung edema, inflammatory cell infiltration and thickening of the alveolar wall were seen in emulsified isoflurane preconditioning group (Grade 2, Fig. 1D), suggesting that lung injury induced by hepatic I/R was attenuated by emulsified isoflurane preconditioning.
Lung tissue W/D weight ratio (n = 8). Emulsified isoflurane suppressed the increases of the lung W/D ratio significantly, while no similar protective effect was observed in NS or lipid vehicle preconditioning. a p<0.01 vs sham group; b p <0.05 vs I/R+S group or I/R+V group.
The lung W/D ratio (a parameter of pulmonary edema) increased significantly in the I/R+S, I/R+V and I/R+E groups compared with that in sham group (Fig. 2). Emulsified isoflurane suppressed the increases of the lung W/D ratio significantly, while no similar protective effect was observed in NS or lipid vehicle preconditioning.
Neutrophil recruitment in the lung was assessed by measuring tissue MPO content. Lung tissue MPO was low in sham rats(1.41±0.51 U/g), but increased to 5.5±1.37, 5.22±1.33 and 3.81±1.62 U/g in I/R+S, I/R+V and I/R+E groups 4 h after hepatic reperfusion (P<0.01). MPO activity in I/R+E was significantly lower than that in I/R+S or I/R+V group (P<0.05, Fig. 3).
Lung tissue MPO activity (n = 8). Lung tissue MPO was low in sham rats and increased in I/R+S, I/R+V and I/R+E groups, while MPO activity in I/R+E was significantly lower than that in I/R+S or I/R+V group. a p<0.01 vs sham group; b p <0.05 vs I/R+S group or I/R+V group.
Compared with sham group, both serum and lung TNF-α levels increased significantly in I/R+S, I/R+V and I/R+E groups 4 h after reperfusion (P<0.05). Statistic analysis showed that both serum and lung TNF-α levels in I/R+E group were significantly lower than those of I/R+S or I/R+V group(P<0.05), and there was no significant difference between I/R+S and I/R+V groups (P>0.05, Fig. 4).
RT-PCR analysis revealed that ICAM-1 mRNA expression was hardly detectable in sham group. However, it was up-regulated markedly in the other three groups (Fig. 5). Compared with I/R+S or I/R+V group, emulsified isoflurane preconditioning reduced ICAM-1 mRNA expression significantly.
A low level of p65 subunit of NF-κB was observed in nuclear extracts of the lungs from sham group. As expected, the nuclear localization of p65 increased markedly in I/R+S, I/R+V and I/R+E groups compared with sham group (Fig. 6). As indicated by previous results, p65 expression was significantly reduced by emulsified isoflurane preconditioning, but not by normal saline or lipid vehicle preconditioning (P<0.05).
Effects of emulsified isoflurane pretreatment on TNF-α levels in lung tissue and serum after hepatic I/R in rats (n = 8). Compared with sham group, both serum and lung TNF-α levels increased significantly in I/R+S, I/R+V and I/R+E groups. Serum and lung TNF-α levels in I/R+E group were significantly lower than those of I/R+S or I/R+V group. a p<0.01 vs sham group; b p <0.05 vs I/R+S group or I/R+V group.
RT-PCR analysis of ICAM-1 mRNA expression in the lung after hepatic I/R (n = 6). ICAM-1 mRNA expression was increased markedly in I/R+S, I/R+V and I/R+E groups. Compared with I/R+S or I/R+V group, emulsified isoflurane preconditioning reduced ICAM-1 mRNA expression significantly. a p<0.01 vs sham group; b p <0.05 vs I/R+S group or I/R+V group.
Effects of emulsified isoflurane pretreatment on NF-κB p65 translocation in the lung after hepatic I/R (n = 3). The top panel shows Western blot analysis for NF-kB p65 in nuclear protein extracts from the rat lung. The bottom panel shows relative densitometric units. The average expression level of the sham group data was set to 1.0, and other data were adjusted to this baseline. The nuclear localization of p65 increased markedly in I/R+S, I/R+V and I/R+E groups compared with sham group, but it was significantly reduced by emulsified isoflurane preconditioning. a p<0.01 vs sham group; b p <0.05 vs I/R+S group or I/R+V group.
Ischemia followed by reperfusion injury is associated with a number of clinical disorders, including systemic inflammatory response syndrome (SIRS), multiple organ dysfunction syndrome (MODS) and multiple system organ failure (MOSF). The lung is one of the most important target organs in MODS or MOSF caused by severe injury. It was found that the lung could also be damaged by remote organ injury such as gut and liver I/R injury22. This process is associated with activation of inflammatory reaction, including the increased activity of NF-κB and the increased inflammatory mediators such as TNF-α and ICAM-123.
The results of our study showed that 90-min hepatic ischemia followed by 4-h reperfusion induced significant lung injury, as manifested by evidence of lung edema, PMN infiltration and histological injuries. Moreover, lung injury was associated with inflammation, as indicated by NF-κB translocation, increase of TNF-α levels and MPO activity, and up-regulation of ICAM-1 expression in the lung tissue.
To our knowledge, this is the first study providing the evidence that preconditioning with emulsified isoflurane could attenuate inflammation reaction and ALI induced by hepatic I/R. In the study we used emulsified isoflurane infused at a rate of 8 ml·kg-1·h-1 for 30 min, knowing that this dosage had no significant inhibitory effect on circulation in pentobarbital anesthetized rats as shown by previous experiments24. Our results showed that emulsified isoflurane preconditioning could reduce lung injury induced by hepatic I/R, as represented by decreased NF-κB activity, TNF-α level and MPO activity, and decreased ICAM-1 expression in the lung as well.
Neutrophils are an important component of the inflammatory response that characterizes ALI25. Activated neutrophils, which infiltrate the lungs during endotoxemia, produce cytokines, such as interleukin-1β and TNF-α, and play a key role in the development of ALI by releasing neutrophil proteases and reactive oxygen species26. Previous studies27-29 showed that isoflurane preconditioning could reduce neutrophil accumulation in the myocardium, and that abolishing neutrophils would induce impairment to contractile function in rat hearts in vitro and in vivo. This inhibitory effect of isoflurane on neutrophils was associated with an inhibition on neutrophil superoxide production and adherence to coronary vascular endothelia. In our study, emulsified isoflurane preconditioning significantly reduced neutrophil accumulation in the lung, which may be associated with the protective effect on the lung.
Lung injury induced by hepatic I/R is thought of as a result of liver-derived TNF-α. In fact, blockade of TNF-α by antibody neutralization greatly reduced hepatic I/R induced lung inflammatory injury in rats30. TNF-α can up-regulate neutrophil adhesion molecules in the liver and remote organs, especially intercellular adhesion molecule-1 (ICAM-1), following hepatic I/R, which then plays an important role in tissue neutrophil influx31.
Some experiments suggested that ischemic preconditioning was able to exhibit anti-inflammatory and protective effect in some organs in vitro and in vivo. Isoflurane could down-regulate LPS-induced production of pro-inflammatory cytokines, including TNF-α and IL-1β in rats32. In this study animals were pretreated with 1.4% isoflurane for 30 min before LPS injection. Notably, isoflurane inhalation was associated with a significant reduction of endotoxemia-induced pulmonary TNF-α and IL-1β. The similar protective effect was also observed in a rat model of renal I/R injury33. Our results showed that emulsified isoflurane preconditioning reduced the serum and lung TNF-α levels and ICAM-1 mRNA expression in lung tissue after hepatic I/R, which may be at least partly contribute to reduced lung injury.
After hepatic ischemia and reperfusion, increased TNF-α level in the circulation initiates a mediator cascade in the lung, including neutrophil infiltration and increased pulmonary vascular expression of intercellular adhesion molecule-1. The gene expression of these proinflammatory mediators is controlled at least partly by the transcription factor NF-κB34.
NF-κB is a key transcription factors that plays a key role in inflammatory response and is activated in the lung after hepatic IR. Activation of NF-κB induced expression of a variety of inflammation-related products, including cytokines, chemokines, and adhesion molecules35,36. Increased concentrations of these inflammatory mediators may contribute to lung injury. Previous studies showed that sevoflurane preconditioning and ischemia preconditioning attenuated NF-κB activation and reduced the expression of inflammatory mediators induced by I/R in the heart, thus decreasing myocardial IR injury37. It was found in the present study that emulsified isoflurane preconditioning had a similar effect on NF-κB translocation in the lung. Emulsified isoflurane preconditioning suppressed the activity of NF-κB significantly and reduced the expression of inflammatory mediators including TNF-α and ICAM-1, both of which contribute to the lung injury after hepatic I/R.
This study, together with previous reports, suggest that emulsified isoflurane preconditioning could ameliorate lung edema and neutrophil recruitment, decrease TNF-α level in serum and lung tissue, and down-regulate ICAM-1 by inhibiting activation of NF-κB, which played a key role in inflammatory response and is activated in the lung after hepatic IR.
In conclusion, the present study demonstrated that emulsified isoflurane may also be protective in surgery- or trauma- related organ injuries occurring secondary to hepatic I/R. Emulsified isoflurane reduced lung injury induced by hepatic I/R, as evidenced by amelioration of lung edema and neutrophil recruitment, decreased TNF-α level in the lung tissue and down-regulation of ICAM-1. Zhang et al38 found that emulsified isoflurane preconditioning protected the liver and lung in a rat model of hemorrhagic shock, which might be due to inhibition of cell death and improvement of anti-oxidation in mitochondria. Rao et al13 found that emulsified isoflurane preconditioning reduced myocardial infarct size, plasma lactate dehydrogenase and creatine kinase levels after myocardial ischemia reperfusion in rats as inhaled isoflurane. So we speculate that the protective mechanism of emulsified isoflurane is generalized and not specific to the lung. These results suggest that emulsified isoflurane may prove applicable to the clinical treatment of organ injury caused by hepatic surgery, transplantation or hemorrhagic shock.
The study was supported by the National Natural Science Foundation of China (Grant No. 30700788) and Youth Scholars Foundation of Shanghai Health Bureau (Grant No. 2009Y064).
The authors have declared that no conflict of interest exists.
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Corresponding author: Wei-Feng Yu, Prof., Department of Anesthesia and Intensive Care, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225# Changhai Road, Shanghai 200438, China. Telephone and Fax: +86-21-81875231. E-mail: ywf808com.